Microbial diversity in chronic open wounds
Microbial diversity in chronic open wounds
复制标题
DOI：
10.1111/j.1524-475x.2009.00472.x
复制
发表时间：
2009-03-01
影响因子：
2.9
通讯作者：
Trent, Jonathan D.
中科院分区：
文献类型：
--
作者：
Frank, Daniel N.;Wysocki, Annette;Trent, Jonathan D.
慢性伤口使真皮基质和下层组织暴露于来自身体和周围环境的多种微生物。我们使用分子方法（rRNA基因序列分析）和常规临床培养方法（使用拭子样本）确定了19个慢性伤口的微生物多样性。我们在2，653个rRNA克隆序列中鉴定了93种微生物类型，并发现与其他环境相比，慢性伤口的微生物多样性相对较好地表征，即，95%的序列与已知的人类同源性>97%。总的来说，75%的序列属于四种众所周知的伤口相关细菌类型：葡萄球菌（25%），棒状杆菌（20%），梭菌（18%）和假单胞菌（12%）。大约0.5%的序列（7个重复类型）属于潜在的新物种。单个伤口样本包含4至22种不同类型，但在所有伤口中，只有少数（1至3种）不同类型占主导地位。在超过一半的伤口标本中，聚合酶链反应和培养方法给出了关于存在的微生物的不同多样性和优势信息。这项探索性研究表明，结合分子和培养方法可以更完整地表征慢性伤口的微生物多样性，从而扩大我们对微生物学如何影响慢性伤口病理和愈合的理解。
Chronic wounds expose the dermal matrix and underlying tissue to a diversity of microbes from the body and surrounding environment. We determined the microbial diversity of 19 chronic wounds using both molecular methods ( sequence analysis of rRNA genes) and routine clinical culturing methods using swab samples. We identified 93 phylotypes in 2,653 rRNA clone sequences and found that compared with other environments, the microbial diversity of chronic wounds is relatively well characterized, i.e., 95% of sequences have >97% identity with known human commensals. In total, 75% of sequences belonged to four well-known wound-associated phylotypes: Staphylococcus (25%), Corynebacterium (20%), Clostridiales (18%), and Pseudomonas (12%). Approximately 0.5% of sequences ( seven phylotypes) belonged to potentially new species. Individual wound samples contained four to 22 phylotypes, but in all wounds only a few ( one to three) phylotypes were dominant. In more than half the wound specimens, polymerase chain reaction and culturing methods gave different diversity and dominance information about the microbes present. This exploratory study suggests that combining molecular and culturing methods provides a more complete characterization of the microbial diversity of chronic wounds, and can thereby expand our understanding of how microbiology impacts chronic wound pathology and healing.