Inactivation of two newly identified tobacco heavy metal ATPases leads to reduced Zn and Cd accumulation in shoots and reduced pollen germination.
Inactivation of two newly identified tobacco heavy metal ATPases leads to reduced Zn and Cd accumulation in shoots and reduced pollen germination.
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两种新鉴定的烟草重金属ATPases的失活导致Zn和CD在芽中的积累减少，并减少了花粉发芽。
DOI：
10.1039/c4mt00071d
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发表时间：
2014-08
期刊：
影响因子：
--
通讯作者：
Berthomieu P
中科院分区：
文献类型：
--
作者：
Hermand V;Julio E;Dorlhac de Borne F;Punshon T;Ricachenevsky FK;Bellec A;Gosti F;Berthomieu P
镉（Cd）是一种非必需的重金属，被国际癌症研究机构（IARC）列为“已知的人类致癌物”。了解Cd在植物体内分布的控制机制对于开发植物修复方法以及食品安全至关重要。与大多数其他植物不同，烟草（Nicotiana tabacum）植物将大部分从土壤中吸收的Cd从根部转移到地上部，导致烟草地上部中的高Cd积累。在烟草中鉴定了拟南芥HMA 2和HMA 4锌和镉ATP酶的两个直系同源物，这些ATP酶负责锌（Zn）和镉从根向芽的转运，并进行了测序。NtHMAα和NtHMAβ基因在根中的表达量高于茎。NtHMAα在根、叶和花药的维管组织中均有表达。在野生型植物和NtHMAα和NtHMAβ基因突变或沉默的植物之间没有观察到视觉差异。这些突变体表现出减少锌和镉的积累，以及增加镉的耐受性。当两个NtHMA基因被沉默，植物发育被改变，花粉萌发严重受损，由于锌缺乏。有趣的是，来自这些品系的种子也显示出降低的Zn浓度，但增加的铁（Fe）浓度。
Cadmium (Cd) is a non-essential heavy metal, which is classified as a “known human carcinogen” by the International Agency for Research on Cancer (IARC). Understanding the mechanisms controlling Cd distribution in planta is essential to develop phytoremediation approaches as well as for food safety. Unlike most other plants, tobacco (Nicotiana tabacum) plants translocate most of the Cd taken up from the soil, out of the roots and into the shoots, leading to high Cd accumulation in tobacco shoots. Two orthologs to the Arabidopsis thaliana HMA2 and HMA4 Zn and Cd ATPases that are responsible for zinc (Zn) and Cd translocation from roots to shoots were identified in tobacco and sequenced. These genes, named NtHMAα and NtHMAβ, were more highly expressed in roots than in shoots. NtHMAα was expressed in the vascular tissues of both roots and leaves as well as in anthers. No visual difference was observed between wild-type plants and plants in which the NtHMAα and NtHMAβ genes were either mutated or silenced. These mutants showed reduced Zn and Cd accumulation in shoots as well as increased Cd tolerance. When both NtHMA genes were silenced, plant development was altered and pollen germination was severely impaired due to Zn deficiency. Interestingly, seeds from these lines also showed decreased Zn concentration but increased iron (Fe) concentration.
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影响因子：
3.5
作者：
Bernard, C;Roosens, N;Verbruggen, N
通讯作者：
Verbruggen, N
影响因子：
13.8
作者：
Korenkov, Victor;King, Brian;Wagner, George J.
通讯作者：
Wagner, George J.
影响因子：
3.7
作者：
Naim F;Nakasugi K;Crowhurst RN;Hilario E;Zwart AB;Hellens RP;Taylor JM;Waterhouse PM;Wood CC
通讯作者：
Wood CC
影响因子：
3.1
作者：
Julio, Emilie;Laporte, Frederic;de Borne, Francois Dorlhac
通讯作者：
de Borne, Francois Dorlhac
DOI：
10.1073/pnas.83.22.8447
发表时间：
1986-11-01
影响因子：
11.1
作者：
JEFFERSON, RA;BURGESS, SM;HIRSH, D
通讯作者：
HIRSH, D