Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection
Expression of the ginseng PgPR10-1 in Arabidopsis confers resistance against fungal and bacterial infection
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DOI：
10.1016/j.gene.2012.06.039
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发表时间：
2012-09-10
期刊：
影响因子：
3.5
通讯作者：
Yang, Deok-Chun
中科院分区：
文献类型：
--
作者：
Lee, Ok Ran;Kim, Yu-Jin;Yang, Deok-Chun
高丽参（Panax ginseng C.）A. Meyer）由来自三个Jakyung、Chungkyung和Hwangsook系的九个品种组成。在三个先前鉴定的人参PR-10同源物（PgPR 10 -1、PgPR 10 -2和PgPR 10 -3）中，我们发现PgPR 10 -2的完全相同的序列存在于所有测试的九个栽培品种中。但在西洋参（Panax quinquefolius）中发现一个缺失和SNP。已知PR-10蛋白是小的和胞质的，并且显示出相似的三维结构。在这里，我们表明，异源过表达的PgPR 10 -1在拟南芥中表现出增强的抗假单胞菌注射，尖孢镰刀菌，灰葡萄孢和框内标记荧光蛋白显示其细胞质和细胞核的定位。PgPR 10 -2与PgPR 10 -1、PgPR 10 -2和PgPR 10 -3的蛋白质-蛋白质相互作用表明，PgPR 10蛋白可能在不同的细胞隔室中形成多聚体复合物，以在发育和防御相关机制中发挥作用。人参植物中PgPR 10 -1和PgPR 10 -2对不同生物胁迫的不同响应支持了这一观点。(C)2012爱思唯尔有限公司版权所有。
Korean ginseng (Panax ginseng C. A. Meyer) consists of nine cultivars from three Jakyung, Chungkyung, and Hwangsook lines. Among three previously identified PR-10 homologs from ginseng (PgPR10-1, PgPR10-2, and PgPR10-3), we found that the exact same sequence of PgPR10-2 exist in all tested nine cultivars. But a deletion and SNP was found in American ginseng (Panax quinquefolius). PR-10 proteins are known to be small and cytosolic, and showed similar three-dimensional structure. Here we show that the heterologous overexpression of PgPR10-1 in Arabidopsis showed enhanced resistance against Pseudomonas syringe, Fusarium oxysporum, and Botrytis cinerea and in-frame tagging with fluorescent protein showed its cytoplasm and nucleus localization. Protein-protein interaction of PgPR10-2 with PgPR10-1, PgPR10-2 and PgPR10-3 suggests that the PgPR10 proteins might form multimeric complexes in different cellular compartments to function in development and in defense-related mechanism. Differential response of PgPR10-1 and PgPR10-2 against different sets of biotic stresses in ginseng plant supports this notion. (C) 2012 Elsevier B.V. All rights reserved.