Quick preparation of genomic DNA from Drosophila.

Quick preparation of genomic DNA from Drosophila.
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DOI:
10.1101/pdb.prot5198
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发表时间:
2009-04-01
影响因子:
--
通讯作者:
Rubin, Gerald M
Rubin, Gerald M
中科院分区:
其他
文献类型:
--
作者:
Huang, Audrey M;Rehm, E Jay;Rubin, Gerald M

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该方案描述了一种用于果蝇的标准小量制备方法,该方法只需要很少的苍蝇,并产生高质量的DNA。当使用无RNA酶条件时,该方法也可用于分离RNA;必须采取额外的步骤来去除样品的基因组DNA(例如,无RNA酶的DNA酶消化)。
This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).