Quick preparation of genomic DNA from Drosophila.
Quick preparation of genomic DNA from Drosophila.
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DOI:
10.1101/pdb.prot5198
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发表时间:
2009-04-01
影响因子:
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通讯作者:
Rubin, Gerald M
中科院分区:
文献类型:
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作者:
Huang, Audrey M;Rehm, E Jay;Rubin, Gerald M
This protocol describes a standard miniprep for Drosophila melanogaster that requires very few flies and produces high-quality DNA. This method can also be used to isolate RNA when RNase-free conditions are utilized; an extra step must be taken to rid the sample of genomic DNA (e.g., RNase-free DNase digestion).