Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells
Intracellular trehalose improves osmotolerance but not desiccation tolerance in mammalian cells
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DOI：
10.1016/s0014-5793(00)02340-1
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发表时间：
2000-12-29
期刊：
影响因子：
3.5
通讯作者：
Tunnacliffe, A
中科院分区：
文献类型：
--
作者：
de Castro, AG;Tunnacliffe, A
海藻糖在一些微生物、无水无脊椎动物和复活植物中起着渗透耐受性或干燥耐受性的作用。为了测试海藻糖是否能改善高等真核生物的应激反应，对小鼠细胞系进行了基因工程改造，使其表达细菌海藻糖合酶基因。我们报道，由此产生的细胞内海藻糖水平（类似于80毫米）能够增强对高渗应激引起的部分脱水的抵抗力，但不能使由于空气干燥而完全干燥的存活。(C) 2000年欧洲生化学会联合会。Elsevier Science b.v.出版，版权所有
Trehalose has been shown to play a role in osmotolerance or desiccation tolerance in some microorganisms, anhydrobiotic invertebrates and resurrection plants. To test whether trehalose could improve stress responses of higher eukaryotes, a mouse cell line was genetically engineered to express bacterial trehalose synthase genes. We report that the resulting levels of intracellular trehalose (similar to 80 mM) are able to confer increased resistance to the partial dehydration resulting from hypertonic stress, but do not enable survival of complete desiccation due to air drying. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V, All rights reserved.