QuEChERS based analysis of multiple pesticides and phthalates in packaged food products
QuEChERS based analysis of multiple pesticides and phthalates in packaged food products
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基于 QuEChERS 的包装食品中多种农药和邻苯二甲酸盐分析
DOI：
10.1016/j.microc.2021.106882
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发表时间：
2021
影响因子：
4.8
通讯作者：
D. Patel
中科院分区：
文献类型：
--
作者：
Juhi Verma;R. R. Jha;Neha Gupta;R. Thakur;N. Ansari;D. Patel
杀虫剂和邻苯二甲酸盐是有毒的化合物，它们可以通过不同的用途存在于我们的食物中。其中一些有毒分析物以痕量形式存在，对于分析化学家来说，尤其是从复杂的食品基质中进行量化一直是一个挑战。开发了一种简单、灵敏、快速、稳健的基于QuEChERS的方法，并对其进行了优化和验证。方法在0.30~50.00 ng/−范围内与(R2)/≥=0.9855呈良好的线性关系。方法的回收率和精密度为71.1-112.5%，−为7.9%，定量限为0.0983-0.8934 ng/g。对合成不确定度和扩展不确定度进行了测定，表明该方法具有很强的分析意义，可以忽略不同的矩阵干扰。该方法成功地用于12个包装谷物样品的分析(有风味的样品=7份，无风味的样品=5份)。采用多元偏最小二乘判别分析方法，分析了调味与非调味谷物样品中农药和邻苯二甲酸酯的含量差异。通过比较折叠变化量(FC)和投影变量重要性分析(VIP)，我们得到了三种农药(S-生物丙烯菊酯、狄氏剂、溴氰菊酯)作为两组间最重要的判别化合物。对FC分析得到的8种农药进行的单因素分析表明，加香料组的农药浓度高于无香料组。拟议的工作将极大地用于这些有毒化合物的常规分析，以应对食品欺诈并揭示消费者面临的相关风险。
Pesticides and phthalates are toxic chemical compounds which could be present in our food items through their different usage. Some of these toxic analytes are present in traces and always a challenge to quantify for analytical chemist especially from complex food matrices. A simple, sensitive, fast and robust QuEChERS based method was developed, optimized and validated. The method showed good linearity in the range of 0.30–50.00 ng g−1with (r2) ≥ 0.9855. The quantification limit was in the range of 0.0983–0.8934 ng g−1with recovery and precision in the range of 71.1–112.5% and RSD < 7.9% for all the analytes. The combined and expanded uncertainty was also determined showing strong analytical significance of the method with negligible matrix interferences. The developed method was successfully used for the analysis of 12 packaged cereal samples (flavoured = 7 & unflavoured = 5). Partial least square discrimination analysis (PLS–DA) of Multivariate approach was used to find differences in profile of pesticides and phthalates between the flavoured and unflavoured cereal samples. We obtained three pesticides (S-BioAllethrin, Dieldrin, Chlorfenapyr) as most important discriminatory compounds for the two groups after comparing fold change (FC) and variable importance in projection (VIP) analysis. Univariate analysis of 8 pesticides obtained from FC analysis showed increased concentration in flavoured group than the unflavoured cereals. The proposed work will be of immense use for routine analysis of these toxic compounds to deal with food fraud and uncover associated risk to the consumer.