Biomechanical characterization and in vitro mechanical injury of elderly human femoral head cartilage:: comparison to adult bovine humeral head cartilage
Biomechanical characterization and in vitro mechanical injury of elderly human femoral head cartilage:: comparison to adult bovine humeral head cartilage
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DOI：
10.1016/j.joca.2005.12.011
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发表时间：
2006-06-01
影响因子：
7
通讯作者：
Quinn, T. M.
中科院分区：
文献类型：
--
作者：
Demarteau, O.;Pillet, L.;Quinn, T. M.
目的：关节软骨的体外机械损伤有助于识别与创伤后骨关节炎（OA）发展相关的事件。迄今为止，许多体外损伤模型都使用动物软骨，尽管人类软骨具有更大的临床意义。我们的目的是表征一种新的老年人类股骨头软骨体外损伤模型，并将其与现有成年牛肱骨头软骨模型的行为进行比较。设计：人类和牛软骨盘的力学性能在径向受限轴压下用弹性模量和水力渗透性来表征，在无侧限压缩下用杨氏模量、泊松比和方向相关径向应变来表征。根据组织水、固体和糖胺聚糖（GAG）含量评估生化组成。在7或70%/s应变速率至3或14 MPa峰值应力的单次损伤斜坡载荷下，通过观察宏观表面组织裂纹和细胞活力的组织学测量来评估机械损伤的反应。结果：老年人股骨软骨的受限压缩模量和杨氏模量比成年牛肱骨软骨大，而其水力渗透性较小。人软骨轴向压缩外植盘的径向变形更具有各向异性（方向依赖性）。在这两种软骨源中，在两种应变速率下的峰值应力为14 MPa时，损伤加载期间组织开裂和相关细胞死亡是常见的。结论：尽管老龄人股骨软骨和成年牛肱骨软骨力学性能不同，但损伤载荷引起的急性损伤相似，支持基于动物的软骨损伤模型的临床意义。然而，固有的结构差异，如细胞密度，可能会影响随后细胞介导的对有害负荷的反应，并影响OA的发展。(c) 2006年国际骨关节炎研究学会。Elsevier Ltd.出版。版权所有。
Objectives: In vitro mechanical injury of articular cartilage is useful to identify events associated with development of post-traumatic osteoarthritis (OA). To date, many in vitro injury models have used animal cartilage despite the greater clinical relevance of human cartilage. We aimed to characterize a new in vitro injury model using elderly human femoral head cartilage and compare its behavior to that of an existing model with adult bovine humeral head cartilage.Design: Mechanical properties of human and bovine cartilage disks were characterized by elastic modulus and hydraulic permeability in radially confined axial compression, and by Young's modulus, Poisson's ratio, and direction-dependent radial strain in unconfined compression. Biochemical composition was assessed in terms of tissue water, solid, and glycosaminoglycan (GAG) contents. Responses to mechanical injury were assessed by observation of macroscopic superficial tissue cracks and histological measurements of cell viability following single injurious ramp loads at 7 or 70%/s strain rate to 3 or 14 MPa peak stress.Results: Confined compression moduli and Young's moduli were greater in elderly human femoral cartilage vs adult bovine humeral cartilage whereas hydraulic permeability was less. Radial deformations of axially compressed explant disks were more anisotropic (direction-dependent) for the human cartilage. In both cartilage sources, tissue cracking and associated cell death during injurious loading was common for 14 MPa peak stress at both strain rates.Conclusion: Despite differences in mechanical properties, acute damage induced by injurious loading was similar in both elderly human femoral cartilage and adult bovine humeral cartilage, supporting the clinical relevance of animal-based cartilage injury models. However, inherent structural differences such as cell density may influence subsequent cell-mediated responses to injurious loading and affect the development of OA. (c) 2006 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.