Bacillus licheniformis MC14 alkaline phosphatase I gene with an extended COOH-terminus.
Bacillus licheniformis MC14 alkaline phosphatase I gene with an extended COOH-terminus.
复制标题
地衣芽孢杆菌 MC14 碱性磷酸酶 I 基因，具有延长的 COOH 末端。
DOI：
10.1111/j.1574-6968.1998.tb12840.x
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发表时间：
1998
影响因子：
2.1
通讯作者：
Hulett,FM
中科院分区：
文献类型：
--
作者：
Kim,JW;Peterson,T;Bee,G;Hulett,FM
除地衣芽孢杆菌外，细菌碱性磷酸酶约为45 kDa的蛋白质，而真核碱性磷酸酶约为60 kDa。为了回答地衣芽孢杆菌的表观60 kDa碱性磷酸酶是否准确地反映了蛋白质的大小的问题，对整个基因进行了分析。碱性磷酸酶I(APaseI)基因序列分析。地衣病毒MC14基因编码553个氨基酸的60 kDa蛋白质。推导的APaseI蛋白序列与OFB蛋白的同源性约为32%。枯草杆菌APase III和IV，在核心结构和活性部位有明显的序列同源性，这些序列在不同来源的APase之间是保守的。APaseI的额外羧基末端序列使该酶比其他原核APase更大，与真核APase的同源性不高。在B的同工酶中，APaseI的氨基酸组成与盐依赖APase的氨基酸组成最为相似。地衣菌属MC14。另一个由261个氨基酸组成的开放阅读框位于APaseI基因上游142个核苷酸，其预测的氨基酸序列与葡萄糖脱氢酶OFB的同源性为68%。巨无霸。
Bacterial alkaline phosphatases (APases), except those isolated fromBacillus licheniformis, are approximately 45-kDa proteins while eucaryotic alkaline phosphatases are 60 kDa. To answer the question of whether the apparent 60-kDa alkaline phosphatase fromBacillus licheniformisaccurately reflected the size of the protein, the entire gene was analyzed. DNA sequence analysis of the alkaline phosphatase I (APaseI) gene ofB. licheniformisMC14 indicated that the gene could code for a 60-kDa protein of 553 amino acids. The deduced protein sequence of APaseI showed about 32% identity to those ofB. subtilisAPase III and IV and had apparent sequence homologies in the core structure and active sites that are conserved among APases of various sources. The extra carboxy-terminal sequence of APaseI, which made the enzyme bigger than other procaryotic APases, was not homologous to those of eucaryotic APases. The amino acid composition of APaseI was most similar to that of salt-dependent APase among the isozymes ofB. licheniformisMC14. Another open reading frame of 261 amino acids was present 142 nucleotide upstream of the APaseI gene and its predicted amino acid sequence showed 68% identity to that of glucose dehydrogenase ofB. megaterium.