Human group-specific component (Gc) is a member of the albumin family.
Human group-specific component (Gc) is a member of the albumin family.
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DOI：
10.1073/pnas.82.23.7994
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发表时间：
1985-12
影响因子：
11.1
通讯作者：
F. Yang;J. L. Brune;S. Naylor;R. Cupples;K. H. Naberhaus;B. Bowman
中科院分区：
文献类型：
--
作者：
F. Yang;J. L. Brune;S. Naylor;R. Cupples;K. H. Naberhaus;B. Bowman
组特异性成分（Gc）是血浆中主要的维生素D结合蛋白。编码Gc的基因在人类4号染色体上与白蛋白和甲胎蛋白基因连锁。这两个基因以前被证明是从一个较小的祖先基因通过基因内三倍体进化而来的。通过用混合寡核苷酸探针筛选成人肝文库，分离出含有编码Gc的人cDNA的重组质粒。Gc cDNA的表征提供了蛋白质的完整氨基酸序列，并显示与白蛋白和甲胎蛋白的强序列同源性。特别令人感兴趣的是形成白蛋白、甲胎蛋白和Gc中的三重结构域结构的二硫键的保守模式。Gc cDNA被用作体细胞杂交的Southern印迹分析中的探针，以确认Gc基因座位于4号染色体上。
The group-specific component (Gc) is the major vitamin D-binding protein in plasma. The gene encoding Gc is linked, on human chromosome 4, to the albumin and alpha-fetoprotein genes. These two genes previously were shown to have evolved from a smaller ancestral gene by intragenic triplication. Recombinant plasmids containing human cDNA encoding Gc have been isolated by screening an adult human liver library with a mixed oligonucleotide probe. Characterization of Gc cDNA has provided the complete amino acid sequence of the protein and revealed strong sequence homology with albumin and alpha-fetoprotein. Of particular interest is a conserved pattern of disulfide bridges that form the triple-domain structures in albumin, alpha-fetoprotein, and Gc. Gc cDNA was used as a probe in Southern blot analysis of somatic-cell hybrids to confirm that the Gc locus is on chromosome 4.