USE OF CHICKEN TRACHEAL ORGAN-CULTURES FOR ISOLATION AND ASSAY OF AVIAN INFECTIOUS BRONCHITIS VIRUS
USE OF CHICKEN TRACHEAL ORGAN-CULTURES FOR ISOLATION AND ASSAY OF AVIAN INFECTIOUS BRONCHITIS VIRUS
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DOI：
10.1007/bf01318005
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发表时间：
1976-01-01
影响因子：
2.7
通讯作者：
PETERS, RW
中科院分区：
文献类型：
--
作者：
COOK, JKA;DARBYSHIRE, JH;PETERS, RW
研究了利用鸡气管器官培养从自然感染和实验感染的鸡中分离和检测禽传染性支气管炎（AIB）病毒。调查了6株AIB病毒，其中3株是从自然暴发的疾病中分离出来的。从AIB爆发中分离出的两种病毒直接进入气管器官培养，而没有在胚卵中传代。使用20日龄的胚胎制备的器官培养物，因为它们在病毒检测中比31日龄的鸡的器官培养物更敏感。作为传染性标志的纤毛感染在接种后3天完成。在气管器官培养物中，可以从呼吸道和非呼吸道组织中分离到病毒，这些培养物在病理物质或连续稀释中检测AIB病毒的灵敏度至少与9日龄胚卵相当。当在两种系统中检测病毒时，滴度非常相似。鸡胚气管器官培养似乎为研究AIB病毒提供了一个可靠的替代系统。
A study was made of the use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis (AIB) virus from naturally and experimentally infected chickens. Six strains of AIB virus were investigated, 3 of which were isolated from natural outbreaks of disease. Two of the virus isolations from the outbreaks of AIB were made directly into tracheal organ cultures without passage in embryonated eggs. Organ cultures prepared from 20 day old embryos were used since they were somewhat more sensitive in virus assay than those derived from chickens of up to 31 days of age. Ciliostasis, used as the marker of infectivity, was complete by 3 days after inoculation with each strain of virus examined. Virus could be isolated from respiratory and non-respiratory tissue in tracheal organ cultures and these cultures were at least sensitive as 9 day old embryonated eggs in detecting AIB virus in pathological material or in serial dilutions. When virus was assayed in both systems, the titers were very similar. Chicken embryo tracheal organ cultures, seem to offer a reliable alternative system to embryonated eggs for studying AIB virus.