THE DNA-BINDING DOMAIN OF HIV-1 INTEGRASE HAS AN SH3-LIKE FOLD
THE DNA-BINDING DOMAIN OF HIV-1 INTEGRASE HAS AN SH3-LIKE FOLD
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DOI：
10.1038/nsb0995-807
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发表时间：
1995-09-01
期刊：
影响因子：
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通讯作者：
HARD, K
中科院分区：
文献类型：
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作者：
EIJKELENBOOM, APAM;LUTZKE, RAP;HARD, K
我们用核磁共振波谱确定了HIV-1整合酶DNA结合区的溶液结构。在溶液中，整合酶的这个羧基末端区域形成一个同源二聚体，由两个非常类似于Src-Homology 3(SH3)结构域的结构组成。先前通过诱变研究确定的Lys 264对整合酶的DNA结合很重要，以及邻近的几个碱性氨基酸都暴露在溶剂中，因此，整合酶中SH3样结构域的鉴定为药物设计提供了一个新的潜在靶点。
We have determined the solution structure of the DNA-binding domain of HIV-1 integrase by nuclear magnetic resonance spectroscopy. In solution, this carboxy-terminal region of integrase forms a homodimer, consisting of two structures that closely resemble Src-homology 3 (SH3) domains. Lys 264 previously identified by mutagenesis studies to be important for DNA binding of the integrase, as well as several adjacent basic amino acids are solvent exposed, The identification of an SH3-like domain in integrase provides a new potential target for drug design.