Tanshinone IIa alleviates LPS-induced oxidative stress in dairy cow mammary epithelial cells by activating the Nrf2 signalling pathway
Tanshinone IIa alleviates LPS-induced oxidative stress in dairy cow mammary epithelial cells by activating the Nrf2 signalling pathway
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DOI：
10.1016/j.rvsc.2022.08.008
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发表时间：
2022-08-23
影响因子：
2.4
通讯作者：
Cao, Rongfeng
中科院分区：
文献类型：
--
作者：
Fu, Kaiqiang;Sun, Yuning;Cao, Rongfeng
目的：乳腺炎是世界范围内奶牛最常见的疾病。有证据表明，氧化应激在乳腺炎的发展中起着至关重要的作用。本研究旨在探讨丹参酮Ⅱ a（Tan Ⅱ a）对内毒素（LPS）诱导的奶牛乳腺上皮细胞（CMECs）氧化应激的抗氧化作用。方法和结果：采用检测试剂盒检测丹参酮Ⅱ a（Tan Ⅱ a）对LPS诱导的CMECs中ROS和MDA水平的影响，发现丹参酮Ⅱ a可显著抑制这些因子的上调。此外，我们还发现Tan Ⅱ a可显著逆转LPS诱导的线粒体膜电位下降。此外，Tan Ⅱ a提高了LPS降低的抗氧化酶活性。最后，我们研究了可能的途径，其中Tan Ⅱ a发挥其抗氧化作用，通过qPCR和蛋白质印迹法，发现Tan Ⅱ a显着激活Keap 1/Nrf 2信号通路。结论：这些结果表明，Tan Ⅱ a可能成为一种可能的治疗剂，用于治疗奶牛乳房炎的氧化应激诱导的CMCs。
Objective: Mastitis is the most prevalent disease in dairy cows worldwide. Evidence has emerged that oxidative stress plays a crucial role in the development of mastitis. This study aimed to investigate the antioxidative effects of tanshinone IIa (Tan IIa) on LPS-induced oxidative stress in dairy cow mammary epithelial cells (CMECs).Methods and results: We examined the levels of ROS and MDA in LPS-treated CMECs after supplementation with Tan IIa using detection kits and found that Tan IIa significantly inhibited the upregulation of these factors. In addition, we also found that Tan IIa significantly reversed the decrease in mitochondrial membrane potential induced by LPS. Moreover, Tan IIa improved the activities of antioxidant enzymes, which were decreased by LPS. Finally, we examined the probable pathway in which Tan IIa exerted its antioxidant effects using qPCR and western blotting and found that Tan IIa significantly activated the Keap1/Nrf2 signalling pathway.Conclusion: These results suggest that Tan IIa might become a possible therapeutic agent for the treatment of dairy cow mastitis by weakening oxidative stress induced by LPS in CMECs.