Novel Activities of Glycolytic Enzymes in Bacillus subtilis
Novel Activities of Glycolytic Enzymes in Bacillus subtilis
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DOI：
10.1074/mcp.m800546-mcp200
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发表时间：
2009-06-01
影响因子：
7
通讯作者：
Stuelke, Joerg
中科院分区：
文献类型：
--
作者：
Commichau, Fabian M.;Rothe, Fabian M.;Stuelke, Joerg
糖酵解是异养生物最重要的代谢途径之一。编码糖酵解酶的几个基因在许多细菌中是必需的，即使在既不需要糖酵解也不需要产酶活性的条件下。在这项研究中，筛选在体内相互作用的合作伙伴的土壤细菌枯草芽孢杆菌的糖酵解酶的糖酵解酶的必要性提供了一个理论基础。糖酵解酶被证明与其他几种蛋白质密切接触，其中有很高比例的必需蛋白质。在这些重要的相互作用伙伴，其他糖酵解酶是最突出的。双杂交研究证实了磷酸果糖激酶与磷酸甘油变位酶和烯醇化酶的相互作用。这种糖酵解酶的复合物可能允许糖酵解中间体的直接底物通道。此外，我们发现糖酵解酶与已知或怀疑参与RNA加工和降解的几种蛋白质的关联。这些蛋白质之一，Rny（YmdA），这是迄今为止还没有被功能上的特点，是所需的糖酵解gapA操纵子的mRNA的加工。双杂交分析证实了糖酵解酶磷酸果糖激酶和烯醇化酶和参与RNA加工的酶，RNase J1，Rny和多核苷酸磷酸化酶之间的相互作用。此外，RNase J1与其同源物RNase J2相互作用。我们认为这种mRNA加工和糖酵解酶的复合体是B。枯草杆菌等同的RNA降解体。我们的研究结果表明，糖酵解酶与必需蛋白质的功能相互作用可能是它们不可或缺的原因。Molecular & Cellular Proteomics 8：1350-1360，2009.
Glycolysis is one of the most important metabolic pathways in heterotrophic organisms. Several genes encoding glycolytic enzymes are essential in many bacteria even under conditions when neither glycolytic nor gluconeogenic activities are required. In this study, a screening for in vivo interaction partners of glycolytic enzymes of the soil bacterium Bacillus subtilis was used to provide a rationale for essentiality of glycolytic enzymes. Glycolytic enzymes proved to be in close contact with several other proteins, among them a high proportion of essential proteins. Among these essential interaction partners, other glycolytic enzymes were most prominent. Two-hybrid studies confirmed interactions of phosphofructokinase with phosphoglyceromutase and enolase. Such a complex of glycolytic enzymes might allow direct substrate channeling of glycolytic intermediates. Moreover we found associations of glycolytic enzymes with several proteins known or suspected to be involved in RNA processing and degradation. One of these proteins, Rny (YmdA), which has so far not been functionally characterized, is required for the processing of the mRNA of the glycolytic gapA operon. Two-hybrid analyses confirmed the interactions between the glycolytic enzymes phosphofructokinase and enolase and the enzymes involved in RNA processing, RNase J1, Rny, and polynucleotide phosphorylase. Moreover RNase J1 interacts with its homologue RNase J2. We suggest that this complex of mRNA processing and glycolytic enzymes is the B. subtilis equivalent of the RNA degradosome. Our findings suggest that the functional interaction of glycolytic enzymes with essential proteins may be the reason why they are indispensable. Molecular & Cellular Proteomics 8: 1350-1360, 2009.