Clinical significance of HAX-1 expression in laryngeal carcinoma
Clinical significance of HAX-1 expression in laryngeal carcinoma
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DOI：
10.1016/j.anl.2014.12.003
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发表时间：
2015-08-01
影响因子：
1.7
通讯作者：
Cao, Xiaolei
中科院分区：
文献类型：
--
作者：
You, Yiwen;Yao, Hao;Cao, Xiaolei
目的：HS1相关蛋白X-1(Hax-1)是一种多功能蛋白，在多种肿瘤中被认为是一种重要的标志物。然而，人们对HAX-1在喉癌中的作用知之甚少。本研究的目的是探讨喉癌组织中HAX-1的表达状况及其与临床病理特征和生存期的关系。方法：采用免疫组织化学、Western blotting和两步实时定量聚合酶链式反应检测HAX-1在喉癌组织和癌旁组织中的表达。分析HAX-1的表达水平与临床病理特征的关系。HAX-1的过表达与T分期、淋巴转移、临床分期和病理类型显著相关。生存曲线用Kaplan-Meier法绘制，用对数列检验进行比较。我们发现，HAX-1过度表达的患者总体存活率较低。最后，用多因素Cox比例风险模型分析各生存变量的显著性。我们发现HAX-1的过度表达是喉癌患者的一个独立的预后因素。结论：HAX-1的过度表达可能是喉癌进展和预后的一个潜在的不利因素。(C)2014爱思唯尔爱尔兰有限公司。保留所有权利。
Objective: HS1-associated protein X-1 (HAX-1) is a multifunctional protein that has been highlighted as an important marker in many types of cancers. However, little is known about the role of HAX-1 in laryngeal carcinoma. The purpose of the present study is to explore HAX-1 expression status and its associations with clinicopathologic features and survival in a well-defined cohort of laryngeal carcinoma.Methods: We examined the expression of HAX-1 at protein and mRNA levels in laryngeal carcinoma tissues and adjacent non-tumor tissues by immunohistochemistry, Western blotting and two-step quantitative real-time PCR analysis, respectively.Results: We observed that HAX-1 was significantly elevated in laryngeal carcinoma. The relationship between the levels of HAX-1 expression and clinicopathologic characteristics was then analyzed. Overexpression of HAX-1 was significantly correlated with T classification, lymph node metastasis, clinical stage, and pathology. Survival curves were plotted using the Kaplan-Meier method and compared using the log-rank test. We find that patients with overexpression of HAX-1 had shorter overall survival rates. Finally, the significance of various survival variables was analyzed using multivariate Cox proportional hazards model. We found that overexpression of HAX-1 was an independent prognostic factor for patients with laryngeal carcinoma.Conclusion: Our findings hinted that overexpression of HAX-1 was a potentially unfavorable factor in the progression and prognosis of laryngeal carcinoma. (C) 2014 Elsevier Ireland Ltd. All rights reserved.