The therapeutic effect of CD133+ cells derived from human umbilical cord blood on neonatal mouse hypoxic-ischemic encephalopathy model
The therapeutic effect of CD133+ cells derived from human umbilical cord blood on neonatal mouse hypoxic-ischemic encephalopathy model
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DOI：
10.1016/j.lfs.2016.06.004
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发表时间：
2016-07-15
期刊：
影响因子：
6.1
通讯作者：
Kudo, Yoshiki
中科院分区：
文献类型：
--
作者：
Kidani, Yukie;Miki, Yasuo;Kudo, Yoshiki
目的：出生时的脑损伤可能导致终生神经发育缺陷。最近，干细胞疗法已应用于多个医学领域。我们之前报道过，CD133(+)细胞（源自人脐带血的内皮祖细胞）在离体缺氧缺血性脑病模型中诱导神经延伸。本实验采用体内模型研究CD133(+)细胞在新生儿缺氧缺血性脑病中的作用。主要方法：采用Rice-Vannucci法诱导新生严重联合免疫缺陷小鼠缺氧缺血性脑损伤。损伤后24小时通过腹腔注射给予CD133(+)细胞。主要发现：免疫组织化学分析显示，腹腔内移植的CD133(+)细胞在注射后48小时向大脑迁移。此外，与未治疗的动物相比，CD133(+)细胞治疗的动物运动功能得到改善，大脑免受缺氧缺血性损伤。意义：我们的结果表明，源自人脐带血的CD133(+)细胞对新生儿缺氧缺血性脑病具有治疗潜力。 (C) 2016 Elsevier Inc. 保留所有权利。
Aims: Brain damage at birth can cause lifelong neurodevelopmental deficits. Recently, stem cell therapies have been used in several fields of medicine. We previously reported that CD133(+) cells, endothelial progenitor cells derived from human umbilical cord blood, induce nerve extension in an ex vivo hypoxic-ischemic encephalopathy model. Here, we used an in vivo model to examine the effect of CD133(+) cells in neonatal hypoxic-ischemic encephalopathy.Main methods: Hypoxic-ischemic brain lesions were induced in neonatal severe combined immunodeficiency mice using the Rice-Vannucci method. CD133(+) cells were administered by intraperitoneal injection 24 h after injury.Key findings: Immunohistochemical analysis revealed that intraperitoneally transplanted CD133(+) cells migrate towards the brain 48 h after injection. Moreover, in CD133(+) cell-treated animals, motor function improved and the brain was protected from the hypoxic-ischemic insult compared with untreated animals.Significance: Our results suggest that CD133(+) cells derived from human umbilical cord blood have therapeutic potential in neonatal hypoxic-ischemic encephalopathy. (C) 2016 Elsevier Inc. All rights reserved.