Automated image analysis of placental villi and syncytial knots in histological sections
Automated image analysis of placental villi and syncytial knots in histological sections
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DOI：
10.1016/j.placenta.2017.04.004
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发表时间：
2017-05-01
期刊：
影响因子：
3.8
通讯作者：
Sharony, Reuven
中科院分区：
文献类型：
--
作者：
Kidron, Debora;Vainer, Ifat;Sharony, Reuven
简介：在组织切片中诊断的绒毛成熟延迟和绒毛成熟加速是病理生理条件的形态学表现。病理学家在评估这些情况时的观察者间一致性充其量是中等的。我们研究了是否自动化图像分析胎盘绒毛和合胞体结可以提高标准化诊断这些conditions.Methods：胎盘产前胎儿死亡或近期被诊断为正常，延迟或加速绒毛成熟。每组5例的组织切片在X10放大倍数下拍照。使用ImageJ公共领域软件进行绒毛和合胞体结的自动图像分析。数百个组织学图像的分析进行了几分钟内在个人电脑上，使用宏命令。结果：正常胎盘相比，绒毛延迟成熟更大，更少，更少，更小的合胞体结。加速成熟的绒毛较小。数据进行了进一步分析，根据水平胎盘区和组的绒毛size.Discussion：正常胎盘可以区分胎盘的绒毛成熟延迟或加速使用自动图像分析。绒毛和合胞体结的自动图像分析不等同于人眼的解释。每种方法在评估代表复杂的三维绒毛树的二维组织学切片时各有优缺点。胎盘的图像分析提供了定量数据，可能有助于为诊断和研究目的对胎盘进行标准化和分级。(C)2017爱思唯尔有限公司版权所有
Introduction: Delayed villous maturation and accelerated villous maturation diagnosed in histologic sections are morphologic manifestations of pathophysiological conditions. The inter-observer agreement among pathologists in assessing these conditions is moderate at best. We investigated whether automated image analysis of placental villi and syncytial knots could improve standardization in diagnosing these conditions.Methods: Placentas of antepartum fetal death at or near term were diagnosed as normal, delayed or accelerated villous maturation. Histologic sections of 5 cases per group were photographed at X10 magnification. Automated image analysis of villi and syncytial knots was performed, using ImageJ public domain software. Analysis of hundreds of histologic images was carried out within minutes on a personal computer, using macro commands.Results: Compared to normal placentas, villi from delayed maturation were larger and fewer, with fewer and smaller syncytial knots. Villi from accelerated maturation were smaller. The data were further analyzed according to horizontal placental zones and groups of villous size.Discussion: Normal placentas can be discriminated from placentas of delayed or accelerated villous maturation using automated image analysis. Automated image analysis of villi and syncytial knots is not equivalent to interpretation by the human eye. Each method has advantages and disadvantages in assessing the 2-dimensional histologic sections representing the complex, 3-dimensional villous tree. Image analysis of placentas provides quantitative data that might help in standardizing and grading of placentas for diagnostic and research purposes. (C) 2017 Elsevier Ltd. All rights reserved.