Explant culture of mouse embryonic whole lung, isolated epithelium, or mesenchyme under chemically defined conditions as a system to evaluate the molecular mechanism of branching morphogenesis and cellular differentiation.
Explant culture of mouse embryonic whole lung, isolated epithelium, or mesenchyme under chemically defined conditions as a system to evaluate the molecular mechanism of branching morphogenesis and cellular differentiation.
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DOI：
10.1007/978-1-59745-019-5_5
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发表时间：
2010
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肺的原始规范和分支形态发生以及各种肺细胞系的形成，需要肺内胚层与其周围的间质和间皮层的特定相互作用。肺间质是肺分支形态发生的诱导信号来源。上皮-间质-间皮的相互作用也是胚胎肺形态发生的关键。早期胚胎肺器官培养是研究上皮-间质相互作用的一个非常有用的系统。上皮细胞和间充质细胞的形态发生都是在特定的条件下进行的，这些条件在这个系统中很容易被操纵（在没有母体影响和血流的情况下）。更重要的是，这项技术可以很容易地在无血清、化学定义的培养基中进行。功能的获得和丧失可以通过表达蛋白、重组病毒载体和/或转基因小鼠品系分析、反义RNA以及RNA干扰基因敲低来实现。此外，为了进一步研究上皮-间充质相互作用，也可以通过组织重组（例如上皮和间充质分离）和微珠研究来确定上皮和间充质信号的相对作用。
Lung primordial specification as well as branching morphogenesis, and the formation of various pulmonary cell lineages, requires a specific interaction of the lung endoderm with its surrounding mesenchyme and mesothelium. Lung mesenchyme has been shown to be the source of inductive signals for lung branching morphogenesis. Epithelial–mesenchymal–mesothelial interactions are also critical to embryonic lung morphogenesis. Early embryonic lung organ culture is a very useful system to study epithelial–mesenchymal interactions. Both epithelial and mesenchymal morphogenesis proceed under specific conditions that can be readily manipulated in this system (in the absence of maternal influence and blood flow). More importantly this technique can be readily done in a serumless, chemically defined culture media. Gain and loss of function can be achieved using expressed proteins, recombinant viral vectors, and/or analysis of transgenic mouse strains, antisense RNA, as well as RNA interference gene knockdown. Additionally, to further study epithelial–mesenchymal interactions, the relative roles of epithelium versus mesenchyme signaling can also be determined using tissue recombination (e.g., epithelial and mesenchymal separation) and microbead studies.