Comparative peptidomic profile between human hypertrophic scar tissue and matched normal skin for identification of endogenous peptides involved in scar pathology.
Comparative peptidomic profile between human hypertrophic scar tissue and matched normal skin for identification of endogenous peptides involved in scar pathology.
复制标题
人肥厚性疤痕组织和匹配正常皮肤之间的肽组谱比较，用于鉴定参与疤痕病理学的内源性肽
DOI：
10.1002/jcp.26407
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发表时间：
2018
影响因子：
5.6
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Li JY;Chen L;Li Q;Cao J;Gao YL;Li J
内源性肽因其参与多种生物学过程而受到越来越多的关注。本研究采用液相色谱-串联质谱法（liquid chromatography-tandem mass spectrometry，LC-MS）对人增生性瘢痕组织和正常皮肤中的肽组进行了比较分析。结果显示，在人增生性瘢痕组织中有179个肽段表达差异显著，其中95个肽段表达上调，84个肽段表达下调。进一步的生物信息学分析（Gene ontology和京都基因百科全书和基因组通路分析）表明，这些差异表达肽的前体蛋白与增生性瘢痕病理变化中发生的细胞过程、生物调节、细胞部分、结合和结构分子活性核糖体以及.通过预测数据库，我们发现78个差异表达肽与抗菌肽和5个已知的免疫调节肽具有同源性，因此我们的研究结果表明，这些肽在人增生性瘢痕组织中的表达谱发生了显著改变。这些肽可能参与了增生性瘢痕的发病机制，为瘢痕的评价和治疗提供了有益的方案。
Endogenous peptides recently attract increasing attention for their participation in various biological processes. Their roles in the pathogenesis of human hypertrophic scar remainspoorly understood.Inthis study, weusedliquid chromatography-tandem mass spectrometry to construct a comparative peptidomic profiling between human hypertrophic scar tissue and matched normal skin. A total of 179 peptides were significantly differentially expressed in human hypertrophic scar tissue, with 95 upregulated and 84 downregulated peptides between hypertrophic scar tissue and matched normal skin. Further bioinformatics analysis (Gene ontology and Kyoto Encyclopedia of Genes and Genomes pathway analysis) indicated that precursor proteins of these differentially expressed peptides correlate with cellular process, biological regulation, cell part, binding and structural molecule activity ribosome, and.PPAR signaling pathway occurring during pathological changes of hypertrophic scar. Based on prediction database, we found that 78 differentially expressed peptides shared homology with antimicrobial peptides and five matched known immunomodulatory peptides.In conclusion, our results show significantly altered expression profiles of peptides in human hypertrophic scar tissue. These peptides may participate in the etiology of hypertrophic scar and provide beneficial scheme for scar evaluation and treatments.