Prolactin inhibits apoptosis of ovarian carcinoma cells induced by serum starvation or cisplatin treatment
Prolactin inhibits apoptosis of ovarian carcinoma cells induced by serum starvation or cisplatin treatment
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DOI：
10.1002/ijc.20810
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发表时间：
2005-07-01
影响因子：
6.4
通讯作者：
Hirahara, F
中科院分区：
文献类型：
--
作者：
Asai-Sato, M;Nagashima, Y;Hirahara, F
催乳素是一种对生殖器官的发育和功能至关重要的肽类激素，与乳腺癌、前列腺癌和结肠直肠癌的发生有关。然而，催乳素在卵巢癌发生中的作用尚不清楚。在本研究中，我们发现催乳素受体的mRNA表达在5的9个卵巢癌细胞系，15的17例手术标本和所有的正常卵巢表面上皮，而催乳素转录只检测到卵巢癌细胞系和手术标本的I。对于催乳素受体阳性的卵巢癌细胞，外源性催乳素不影响其增殖，但明显抑制其凋亡。因此，催乳素以剂量依赖性方式增强实际细胞生长。抗体对催乳素受体的阻断严重影响了催乳素的抗凋亡和促生长作用。有趣的是，用催乳素预处理可显著抑制催乳素受体阳性细胞的顺铂诱导的细胞死亡。提示催乳素/催乳素受体系统可能成为卵巢癌治疗的新靶点。(c)2005年威利-利斯。Inc.
Prolactin, a peptide hormone essential for the development and function of reproductive organs, is involved in development of breast, prostate and colorectal cancers. However, the role of prolactin on the carcinogenesis of ovarian carcinomas is unclear. In this study, we show that mRNA of prolactin receptor is expressed in 5 out of 9 ovarian carcinoma cell lines, 15 out of 17 cases of surgical samples and all of normal ovarian surface epithelium, while prolactin transcript is detected only in I ovarian carcinoma cell line and in I of the surgical samples. For the prolactin receptor-positive ovarian carcinoma cells, exogenous prolactin did not affect the proliferation but markedly inhibited apoptosis. Therefore, actual cell growth was enhanced by prolactin in a dose-dependent manner. The blocking of prolactin receptor by antibody severely impaired the antiapoptotic and growth-promoting effects of prolactin. Interestingly, the cisplatin-induced cell death of the prolactin receptor-positive cells was significantly inhibited by pretreatment with prolactin. These findings indicate a responsiveness of ovarian carcinomas to prolactin and suggest that the prolactin/prolactin receptor system may be a new therapeutic target of ovarian carcinomas. (c) 2005 Wiley-Liss. Inc.