Auxin-Induced Plasma Membrane Depolarization Is Regulated by Auxin Transport and Not by AUXIN BINDING PROTEIN1
Auxin-Induced Plasma Membrane Depolarization Is Regulated by Auxin Transport and Not by AUXIN BINDING PROTEIN1
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DOI：
10.3389/fpls.2018.01953
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发表时间：
2019-01-17
影响因子：
5.6
通讯作者：
Palme, Klaus
中科院分区：
文献类型：
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作者：
Paponov, Ivan A.;Dindas, Julian;Palme, Klaus
生长素是一种通过转录和非转录信号反应控制植物发育许多方面的分子。生长素结合蛋白1 （ABP1）被认为是生长素诱导的质膜去极化和内吞速率快速非转录变化的受体。然而，abp1介导的信号传导机制尚不清楚。本研究表明，膜去极化和内吞抑制是与abp1无关的反应，而生长素诱导的质膜去极化依赖于生长素内流载体AUX1。AUX1本身不参与胞吞作用的调节。生长素依赖的质膜去极化也被生长素外排载体PIN2调节。这些数据在生长素运输和非转录生长素信号传导之间建立了新的联系。
Auxin is a molecule, which controls many aspects of plant development through both transcriptional and non-transcriptional signaling responses. AUXIN BINDING PROTEIN1 (ABP1) is a putative receptor for rapid non-transcriptional auxin-induced changes in plasma membrane depolarization and endocytosis rates. However, the mechanism of ABP1-mediated signaling is poorly understood. Here we show that membrane depolarization and endocytosis inhibition are ABP1-independent responses and that auxin-induced plasma membrane depolarization is instead dependent on the auxin influx carrier AUX1. AUX1 was itself not involved in the regulation of endocytosis. Auxin-dependent depolarization of the plasma membrane was also modulated by the auxin efflux carrier PIN2. These data establish a new connection between auxin transport and non-transcriptional auxin signaling.