Lack of B cell leakiness in BALB/cA-nu, scid double mutant mice
Lack of B cell leakiness in BALB/cA-nu, scid double mutant mice
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DOI：
10.1538/expanim.50.67
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发表时间：
2001-01-01
影响因子：
2.4
通讯作者：
Ito, M
中科院分区：
文献类型：
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作者：
Hioki, K;Kuramochi, T;Ito, M
通过BALB/cA-scid与BALB/cA-nu的杂交，获得nu和scid突变纯合的BALB/cA小鼠（BALB/cA-nu/nu, scid/scid）。这些小鼠比C.B-17-scid寿命更长，因为它们不像后者那样发生胸腺淋巴瘤。C.B-17-scid已知表现出泄漏现象，在衰老的C.B-17-scid中出现了一些功能性T细胞和B细胞克隆。出乎意料的是，当用RT-PCR检测细胞因子表达、用流式细胞术检测淋巴细胞表型和ELISA检测血清免疫球蛋白水平时，BALB/cA-nu - scid小鼠中漏出的B细胞和T细胞不存在或被抑制。这些结果表明B细胞渗漏可能是由泄漏的T细胞诱导的。BALB/cA-nu， scid小鼠可能在同种和异种移植实验中作为有用的受体，因为除了没有毛发外，没有胸腺淋巴瘤和渗漏。
BALB/cA mice homozygous for both nu and scid mutations (BALB/cA-nu/nu, scid/scid) were developed by mating between BALB/cA-scid and BALB/cA-nu. These mice have greater longevity than C.B-17-scid because no thymic lymphoma occurs in them unlike in the latter C.B-17-scid is known to show the leaky phenomenon in which a few clones of functional T and B cells develop in aged C.B-17-scid. Unexpectedly, the leaky B cells and T cells were absent or suppressed in BALB/cA-nu, scid mice when cytokine expressions were determined by RT-PCR, lymphocyte phenotypes by flow cytometry and serum immunoglobulin levels by ELISA. These results indicate that B cell leakiness may be induced by leaked T cells. BALB/cA-nu, scid mice may be useful as a recipient in allo- and xeno-transplantation experiments because of the absence of both thymic lymphomas and leakiness, in addition to lack of hair.