Enhancing tyrosine hydroxylase expression and survival of fetal ventral mesencephalon neurons with rat or porcine Sertoli cells in vitro.
Enhancing tyrosine hydroxylase expression and survival of fetal ventral mesencephalon neurons with rat or porcine Sertoli cells in vitro.
复制标题
在体外用大鼠或猪支持细胞增强胎儿腹侧中脑神经元的酪氨酸羟化酶表达和存活。
DOI：
10.1016/j.brainres.2006.04.058
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Willing,AlisonE
中科院分区：
文献类型：
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作者：
Shamekh,Rania;Mallery,Jennifer;Newcomb,Jennifer;Hushen,Joelle;Saporta,Samuel;Cameron,DonF;Sanberg,CyndyDavis;Sanberg,PaulR;Willing,AlisonE
支持细胞（Sertoli cells，SC）是睾丸来源的细胞，分泌对生殖细胞发育重要的营养因子。猪和大鼠SC都已被用作移植促进剂-新生猪SC用于支持糖尿病中的胰岛，15天大的大鼠SC用于增强帕金森病模型中的多巴胺能神经元移植。然而，从来没有一个研究检查最佳的SC制剂，以提高酪氨酸羟化酶在腹侧中脑（VM）神经元的表达。本研究的目的是比较大鼠和猪的SC，以提高酪氨酸羟化酶的表达（TH）和神经元的存活在相同的出生后发育年龄的能力。从1、9或15日龄的大鼠或新生儿（2-5日龄）、2月龄或4月龄的猪中分离SC，并与来自13.5日龄胚胎的VM组织共培养。我们的研究结果表明，VM神经元与SC共培养分散在培养板上，并有广泛的神经突生长，而单独培养的VM神经元往往聚集在一起，形成一个有限的神经突生长的细胞团。当VM神经元与15日龄大鼠SC或2月龄猪SC共培养时，TH表达显著增加，但当细胞与其他年龄的SC共培养时，TH表达没有增加。这表明SC分泌的营养因子根据发育年龄而变化，并且使用来自适当年龄和物种的SC对于移植促进的成功至关重要。
Sertoli cells (SCs) are testis-derived cells that secrete trophic factors important for the development of germ cells. Both porcine and rat SCs have been used as graft facilitators — neonatal porcine SCs to support islets in diabetes and 15-day-old rat SCs to enhance dopaminergic neuron transplants in Parkinson's disease models. However, there has never been a study examining the optimal SCs preparation to enhance tyrosine hydroxylase expression in the ventral mesencephalon (VM) neuron. The aim of this study was to compare the ability of both rat and porcine SCs to enhance tyrosine hydroxylase expression (TH) and neuronal survival at the same postnatal developmental ages. The SCs were isolated from 1-, 9-, or 15-day-old rat, or neonate (2–5 days), 2-month, or 4-month-old pig, and co-cultured with VM tissue from 13.5-day-old embryos. Our results showed that VM neurons co-cultured with SCs dispersed over the culture plate and had extensive neuritic outgrowth, while VM neurons cultured alone tended to cluster together forming a mass of cells with limited neurite outgrowth. TH expression was significantly increased when VM neurons were co-cultured with 15-day rat SCs or 2-month pig SCs but not when the cells were co-cultured with other ages of SCs. This suggests that secretion of trophic factors by SCs varies according to the developmental age, and it is critical for the success of graft facilitation that SCs from the appropriate age and species be used.