Effect of a photosensitization reaction performed during the first 3 min after exposure of rat myocardial cells to talaporfin sodium in vitro
Effect of a photosensitization reaction performed during the first 3 min after exposure of rat myocardial cells to talaporfin sodium in vitro
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DOI：
10.1007/s10103-017-2298-3
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发表时间：
2017-11-01
影响因子：
2.1
通讯作者：
Arai, Tsunenori
中科院分区：
文献类型：
--
作者：
Doi, Marika;Ogawa, Emiyu;Arai, Tsunenori
为了更好地了解光动力心脏消融的机制，我们研究了大鼠心肌细胞暴露于塔拉波芬钠后前3分钟发生的光敏反应（PR）的影响。采用3mm平方的64个微电极阵列连续测量心肌细胞的动作电位。使用30 μ g/mL氯光敏剂塔拉泊芬钠溶液，同时使用663 nm红色二极管激光器（86 mW/cm(2)），持续时间长达600 s。与Na+动力学相对应的动作电位波形第一波谷随PR持续时间的增加呈指数下降。从PR发作开始，指数函数的平均衰减时间为20.1 s。结果表明，微电极阵列测得的动作电位波形行为可以作为一种微创的方法来评价PR对心肌细胞的电生理作用。
To better understand the mechanism of photodynamic cardiac ablation, we studied the effects of a photosensitization reaction (PR) performed during the first 3 min after rat myocardial cells were exposed to talaporfin sodium. A 3-mm-square microelectrode arraywith 64 electrodeswas used to continuously measure the action potentials of the myocardial cells. A 30 mu g/mL talaporfin sodium solution, a chlorine photosensitizer, was used, along with a 663-nm red diode laser (86 mW/cm(2) for up to 600 s). The first trough of the measured action potential waveform corresponding to Na+ dynamics decreased exponentially with increasing PR duration. The average decay time of the exponential function from PR onsetwas 20.1 s. Marked morphological changes in the myocardial cells was observed after the PR. These results indicated that the behavior of the action potential waveform measured by the microelectrode array might be used as a less invasive method to evaluate the electrophysiological effects of a PR on myocardial cells.