In vitro beta-carotene toxicity for human colon cancer cells.
In vitro beta-carotene toxicity for human colon cancer cells.
复制标题
体外 β-胡萝卜素对人类结肠癌细胞的毒性。
DOI：
10.1080/01635589609514446
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Frommel,TO
中科院分区：
文献类型：
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作者：
Iftikhar,S;Lietz,H;Mobarhan,S;Frommel,TO
进行实验以确定β-胡萝卜素对体外人结肠癌细胞的作用。确定溶解于四氢呋喃（THF）中的β-胡萝卜素对三种不同细胞系具有细胞毒性：LS 180、SW 620和HCT-15。2.9 μ g/ml的LS 180和SW 620细胞存活数明显低于对照组（P < 0.05）。相对于THF处理的细胞，β-胡萝卜素显著减少，并且在HCT-15细胞中使用5.8 μ g/ml的β-胡萝卜素也实现了类似的减少。β-胡萝卜素这些浓度在补充30 mg/天β-胡萝卜素的个体血清中达到的范围内。在未补充的个体血清的浓度范围内，β-胡萝卜素无细胞毒性。维生素？在>200 μ？没有细胞毒性，并且在较高浓度下轻微刺激所有三种细胞系的增殖。细胞暴露于维生素？没有降低β-胡萝卜素的细胞毒性，表明β-胡萝卜素的毒性作用不是由于促氧化活性。通过延长细胞暴露于β-胡萝卜素的持续时间来增加细胞毒性百分比。有趣的是，β-胡萝卜素的细胞毒性随着细胞密度的增加而降低。这种密度依赖性毒性可归因于在较低密度下接种的细胞中每个细胞的β-胡萝卜素浓度较高。因此，β-胡萝卜素对结肠癌细胞的毒性是剂量、时间和细胞密度依赖性的，并且在体外可以在人体中安全达到的浓度下发生。
Experiments were conducted to determine the effect of β‐carotene on human colon cancer cells in vitro. β‐Carotene solubilized in tetrahydrofuran (THF) was determined to be cytotoxic for three different cell lines: LS 180, SW620, and HCT‐15. The number of LS 180 and SW 620 cells surviving treatment with 2.9 μ? β‐carotene was significantly reduced relative to THF‐treated cells, and a similar reduction was achieved in HCT‐15 cells with use of 5.8 μ? β‐carotene. These concentrations are in the range achieved in serum of individuals supplemented with β‐carotene at 30 mg/day. There was no β‐carotene cytotoxicity in the concentration range that characterizes serum of unsupplemented individuals. Vitamin ? at >200 μ? was not cytotoxic and at higher concentrations slightly stimulated proliferation of all three cell lines. Exposure of cells to vitamin ? did not diminish the cytotoxicity of β‐carotene, suggesting that the toxic effect of β‐carotene is not due to prooxidant activity. Percent cytotoxicity was increased by extending the duration of exposure of cells to β‐carotene. Interestingly, β‐carotene cytotoxicity decreased with increasing cell density. This density‐dependent toxicity was attributable to a higher β‐carotene concentration per cell for cells plated at lower densities. Thus toxicity of β‐carotene for colon cancer cells is dose, time, and cell density dependent and occurs in vitro at concentrations that can be achieved safely in humans.