Neurobehavioral effects of postnatal exposure to low-level mercury vapor and/or methylmercury in mice.
Neurobehavioral effects of postnatal exposure to low-level mercury vapor and/or methylmercury in mice.
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DOI：
10.2131/jts.43.11
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Minoru Yoshida;Jin-Yong Lee;M. Satoh;C. Watanabe
中科院分区：
文献类型：
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作者：
Minoru Yoshida;Jin-Yong Lee;M. Satoh;C. Watanabe
本研究探讨了低水平汞蒸气(Hg0)、甲基汞(MeHg)对雌性小鼠神经行为功能的影响，以及Hg0和MeHg联合作用对小鼠神经行为功能的影响。出生后第2天至第28天，小鼠暴露于平均浓度为0.188 mg/m~3的Hg0，饲喂含3.85μg/g甲基汞的食物。联合暴露组同时暴露于汞和甲基汞，使用相同的程序。当它们的后代达到11周大时，进行行为分析。以运动活动度和开阔视野中心进入率(OPF)、被动回避反应(PA)学习活动和放射状迷宫(RM)空间学习能力为指标评价其对小鼠行为的影响。与对照组相比，Hg0组、MeHg组和联合暴露组大鼠OPF的总运动活动明显减少。联合暴露组OPF中央区域的条目比例显著高于对照组，而汞或甲基汞暴露组与对照组差异无统计学意义。其他行为测试没有显示出这些群体之间的显著差异。与单独接触汞或甲基汞相比，联合暴露后的行为异常更明显。子代暴露后即刻的脑汞浓度在联合暴露组最高，超过2μg/g，其次是甲基汞和汞暴露组。因此，联合暴露组神经行为效应的增强与较高的脑汞浓度有关。
This study examined the effects on neurobehavioral function of exposure to low-level mercury vapor (Hg0), methylmercury (MeHg) in female mice and the combination of Hg0 and MeHg during postnatal development. Postnatal mice were exposed to Hg0 at a mean concentration of 0.188 mg/m3 Hg0 and supplied with food containing 3.85 μg/g of MeHg from day 2 to day 28 after delivery. The combined exposure group was exposed to both Hg0 and MeHg, using the same procedure. When their offspring reached the age of 11 weeks, behavioral analyses were performed. The behavioral effects in mice were evaluated based on locomotive activity and rate of center entries in the open field (OPF), learning activity in the passive avoidance response (PA) and spatial learning ability in the radial maze (RM). Total locomotive activity in the OPF significantly decreased in the Hg0, MeHg and combined exposure groups compared with the control group. The proportion of entries to central area in the OPF was significantly higher in the combined exposure group than in the control group, while those in the Hg0 or MeHg exposure group did not differ from the control group. Other behavioral tests did not reveal significant differences among the groups. Behavioral anomalies were more distinctive after combined exposure compared to Hg0 or MeHg exposure alone. The brain Hg concentration of offspring, immediately after exposure, was highest in the combined exposure group, exceeding 2 μg/g, followed by the MeHg and Hg0 exposure groups. Thus, the enhancement of neurobehavioral effects in the combined exposure group was associated with higher brain mercury concentration.