Duck immune responses to Riemerella anatipestifer vaccines
Duck immune responses to Riemerella anatipestifer vaccines
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DOI：
10.1016/s0145-305x(99)00070-1
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发表时间：
2000-03-01
影响因子：
2.9
通讯作者：
Kounev, ZV
中科院分区：
文献类型：
--
作者：
Higgins, DA;Henry, RR;Kounev, ZV
鸭疫里默氏杆菌（Riemerella anatipestifer，Ra）感染可能是世界范围内最重要的经济传染病，但对自然感染和疫苗的免疫应答知之甚少。我们使用淋巴细胞转化试验（LTT）研究细胞介导的免疫（CMI）的表达，和酶联免疫吸附试验监测抗体（Ab）生产后，福尔马林灭活和减毒活血清型2（= G）Ra疫苗。用冷冻-解冻和超声处理制备的Ra抗原刺激淋巴细胞（8 × 10（5），溶于200 μ l RPMI +10%鸭血清中，置于96孔板中）;用6.25 μ g/ml抗原获得最佳应答。在评估H-3-胸苷摄取之前，将细胞在41.6 ℃/5%CO2下培养3天。掺入氢氧化铝作为佐剂的Ra蛋白刺激了强烈但短暂（约4周）的LTT应答; LTT与来自其他Ra血清型的蛋白质有一些交叉反应。再接种刺激略强的反应与相同的时间过程。对每次接种的Ab应答比LTT应答持续时间更长。用活的Ra减毒株接种疫苗刺激较弱但持续时间较长的LTT应答，但与Bclarin相比类似的Ab应答。因此，很明显，报告使用Ra-β蛋白的瞬时保护作用是由于对这些疫苗的CMI应答是瞬时的;当LT-T检测不到CMI时，鸭可能具有可检测水平的血清Ab。这些观察结果是重要的，在我们的理解免疫发病机制，免疫预防和免疫诊断RA。(C)2000爱思唯尔科技有限公司版权所有。
Riemerella anatipestifer (Ra) infection is probably the most economically important infectious disease of farm ducks worldwide but the immune responses to natural infection and vaccines are poorly understood. We have used the lymphocyte transformation test (LTT) to study the expression of cell-mediated immunity (CMI), and the enzyme-linked immunosorbent assay to monitor antibody (Ab) production following administration of formalin-inactivated and live attenuated serotype 2 (= G) Ra vaccines. Lymphocytes (8 x 10(5) in 200 mu l of RPMI + 10% duck serum, in 96 well trays) were stimulated with Ra antigen, prepared by freeze-thaw and sonication; optimum responses were obtained with antigen at 6.25 mu g/ml. Cells were cultured for 3 days at 41.6 degrees C/5% CO2, prior to assessing H-3-thymidine uptake. Ra bacterin, incorporating aluminium hydroxide as adjuvant, stimulated strong but transient (about 4 weeks) LTT response; there was some cross-reaction of the LTT to proteins derived from other serotypes of Ra. Revaccination stimulated slightly stronger responses with the same time course. The Ab response to each vaccination was longer-lived than the LTT response. Vaccination with a live, attenuated strain of Ra stimulated weaker but longer lasting LTT responses, but similar Ab responses compared to the bacterin. It is apparent, therefore, that the transient protection reported using Ra bacterins is due to the fact that the CMI response to these vaccines is transient; and that it is possible for ducks to have detectable levels of serum Ab at times when CMI is not detectable by LT-T. These observations are important in terms of our understanding of immunopathogenesis, immunoprophylaxis, and immunodiagnosis in Ra. (C) 2000 Elsevier Science Ltd. All rights reserved.