EFFECT OF CYCLOHEXIMIDE ON THE CELL CYCLE OF THE CRYPTS OF THE SMALL INTESTINE OF THE RAT
EFFECT OF CYCLOHEXIMIDE ON THE CELL CYCLE OF THE CRYPTS OF THE SMALL INTESTINE OF THE RAT
复制标题
放线菌酮对大鼠小肠隐窝细胞周期的影响
作者：
R. Verbin;E. Farber
单次注射1.5 mg/kg放线菌酮可在1.5-2小时内诱导大鼠肠隐窝有丝分裂活性完全消失。尽管这种现象伴随着标记前体摄取蛋白质和DNA的显著减少，但未观察到隐窝细胞的显著坏死。有丝分裂在注射后6小时重新出现，然后在相当于一个细胞周期的时间段内恢复遵循循环模式，从而反映了细胞分裂的至少部分同步化。同时使用秋水仙碱，一种已知诱导中期阻滞的药物，已经证明放线菌酮，虽然对已经分裂的细胞没有明显的影响，但可以阻止新细胞进入可见的有丝分裂。细胞周期的分析表明，放线菌酮引发的一个阻滞发生在G2期，可能是由于干扰了细胞从周期的这一阶段进入有丝分裂所需的蛋白质的形成。
A single injection of 1.5 mg/kg of cycloheximide induces a complete disappearance of mitotic activity in rat intestinal crypts within 1.5–2 hr. No significant necrosis of crypt cells is observed even though this phenomenon is accompanied by a marked decrease in uptake of labeled precursors into protein and DNA. Mitoses reappear 6 hr after injection and recovery then follows a cyclic pattern over a period equivalent to one cell cycle, thereby reflecting at least a partial synchronization of cell division. Concurrent use of colchicine, an agent known to induce metaphase arrest, has demonstrated that cycloheximide, while having no apparent effect on cells already in division, prevents the entrance of new cells into visible mitosis. Analysis of the cell cycle suggests that one block initiated by cycloheximide occurs in G2, presumably as the result of an interference with the formation of protein(s) required for the normal progression of cells from this phase of the cycle into mitosis.