THE MULTIFUNCTIONAL REGULATORY PROTEINS ABF1 AND CPF1 ARE INVOLVED IN THE FORMATION OF A NUCLEASE-HYPERSENSITIVE REGION IN THE PROMOTER OF THE QCR8 GENE
THE MULTIFUNCTIONAL REGULATORY PROTEINS ABF1 AND CPF1 ARE INVOLVED IN THE FORMATION OF A NUCLEASE-HYPERSENSITIVE REGION IN THE PROMOTER OF THE QCR8 GENE
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DOI：
10.1002/yea.320090805
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发表时间：
1993-08-01
期刊：
影响因子：
2.6
通讯作者：
GRIVELL, LA
中科院分区：
文献类型：
--
作者：
DEWINDE, JH;VANLEEUWEN, HC;GRIVELL, LA
ABF 1和CPF 1是酿酒酵母中丰富的DNA结合蛋白，是具有多种染色体功能的全局调节因子家族的成员。最近的证据表明，这些蛋白质因子可能参与建立和维持启动子区域和其他遗传元件中定义明确的染色质结构。我们已经调查了ABF 1和CPF 1在QCR 8基因的染色质组织中的参与，QCR 8基因编码线粒体泛喹啉-细胞色素c氧化还原酶的亚基VIII。QCR 8基因的启动子区含有ABF 1和CPF 1的重叠结合位点。核小体定位研究表明，QCR 8基因与一个相控阵列的核小体在代谢产物抑制和去抑制的生长条件下。结合位点突变体的分析表明，ABF 1和CPF 1都参与维持QCR 8启动子中的核酸酶超敏感区。然而，在稳态生长过程中QCR 8的染色质结构主要依赖于ABF 1与启动子区域的结合。ABF 1和CPF 1在调节线粒体生物发生和其他对细胞生长和分裂重要的过程中所起的作用，这些发现的影响将进行讨论。
The abundant DNA-binding proteins ABF1 and CPF1 are members of a family of global regulators with diverse chromosomal functions in the yeast Saccharomyces cerevisiae. Recent evidence suggests that these protein factors may be involved in establishing and maintaining well-defined chromatin structures in promoter regions and other genetic elements. We have investigated the involvement of ABF1 and CPF1 in chromatin organization at the QCR8 gene, encoding subunit VIII of the mitochondrial ubiquinol-cytochrome c oxidoreductase. The promoter region of the QCR8 gene contains overlapping binding sites for ABF1 and CPF1. Nucleosome positioning studies indicate that the QCR8 gene is associated with a phased array of nucleosomes under both catabolite-repressed and derepressed growth conditions. Analysis of binding site mutants reveals that both ABF1 and CPF1 are involved in maintaining a nuclease-hypersensitive region in the QCR8 promoter. The chromatin structure at QCR8 during steady-state growth is, however, mainly dependent on binding of ABF1 to the promoter region. Implications of these findings for the role played by ABF1 and CPF1 in the regulation of mitochondrial biogenesis and other processes important for cell growth and division will be discussed.