CNE1, A SACCHAROMYCES-CEREVISIAE HOMOLOG OF THE GENES ENCODING MAMMALIAN CALNEXIN AND CALRETICULIN
CNE1, A SACCHAROMYCES-CEREVISIAE HOMOLOG OF THE GENES ENCODING MAMMALIAN CALNEXIN AND CALRETICULIN
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DOI：
10.1002/yea.320090209
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发表时间：
1993-02-01
期刊：
影响因子：
2.6
通讯作者：
WIEMKEN, A
中科院分区：
文献类型：
--
作者：
DEVIRGILIO, C;BURCKERT, N;WIEMKEN, A
与不同物种的钙网蛋白基因具有高度同源性的酿酒酵母的DNA片段（Fliegel等人，1989; Smith和Koch，1989; Murphy等人，1990; Rokeach等人，1991）和犬的钙连接蛋白基因（Wada et al.，1991）从基因组DNA文库中偶然克隆（Rose等，1987年）。它包含一个1506个核苷酸的开放阅读框，编码56.9 kDa的预测基因产物。此外，对5 '非编码区的258个核苷酸和3'非编码区的264个核苷酸进行了测序（图1）。酵母染色体DNA的脉冲场电泳和Southern印迹分析表明该基因定位于染色体I。此外，基于对限制性位点的分析，该基因可以被分配给克隆的插入物hR 23 d（Kaback，1992，personal communication; Kaback et al.，1989年）。推导的氨基酸序列的计算机分析（Devereux等人，1984）揭示了S.酿酒酵母基因产物与酿酒酵母的钙网蛋白的氨基酸序列具有显著的相似性，
A DNA fragment of Saccharomyces cerevisiae with high homology to the calreticulin genes of different species (Fliegel et al., 1989; Smith and Koch, 1989; Murphy et al., 1990; Rokeach et al., 1991) and the calnexin gene of Canis domesticus (Wada et al., 1991) was cloned serendipitously from a genomic DNA library (Rose et al., 1987). It contains an open reading frame of 1506 nucleotides encoding a predicted gene product of 56.9 kDa. In addition, 258 nucleotides at the 5'non-coding region and 264 nucleotides at the 3'non-coding region were sequenced (Figure 1). Pulse-field electrophoresis of yeast chromosomal DNA followed by Southern blot analysis showed the gene to be localized on chromosome I. Furthermore, based on an analysis of restriction sites, the gene could be assigned to the cloned insert hR23d (Kaback, 1992, personal communication; Kaback et al., 1989). Computer analysis of the deduced amino acid sequence (Devereux et al., 1984) revealed that the S. cerevisiae gene product shares significant similarity with the amino acid sequences of the calreticulins of