The application of the supported liquid membrane and molecularly imprinted polymers as solid acceptor phase for selective extraction of biochanin A from urine
The application of the supported liquid membrane and molecularly imprinted polymers as solid acceptor phase for selective extraction of biochanin A from urine
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DOI：
10.1016/j.chroma.2019.04.005
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发表时间：
2019-08-16
影响因子：
4.1
通讯作者：
Martin-Esteban, Antonio
中科院分区：
文献类型：
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作者：
Chrzanowska, Anna M.;Diaz-Alvarez, Myriam;Martin-Esteban, Antonio
建立了一种用于尿液中植物雌激素分析的高效样品净化和预富集方法。采用中空纤维支撑液膜固相萃取和分子印迹微球(MIPs-HF-SLM-SPE)相结合的方法。以生物粘附素A(BCA)为模板，采用沉淀聚合法合成了分子印迹聚合物(MIP)，得到了形状规则的窄分散微球。作为功能单体，甲基丙烯酸二甲氨基乙酯(-DEM)的印迹效果优于甲基丙烯酸(MAA)。与非印迹聚合物及其结构类似物(染料木素和大豆苷元)相比，MIPs-HF-SLM-SPE萃取过程中用作吸附剂的分子印迹聚合物对BCA具有良好的结合选择性。最后，将所建立的方法用于尿中BCA的检测。在最佳提取条件下，尿样(4.5m L尿样中加入10 mU g L-1)中溴乙酸的回收率在41%以上，变异系数为6.6%(n=5)。尿中BCA分析的检出限和定量限分别为0.41和1.36mgl-1。(C)2019爱思唯尔B.V.保留所有权利。
An efficient sample clean-up and preconcentration procedure for phytoestrogens analysis in urine has been developed. It was based on a combination of solid phase extraction with hollow-fiber supported liquid membrane and molecularly imprinted beads (MIPs-HF-SLM-SPE). The molecularly imprinted polymers (MIPs) were synthesized by precipitation polymerization technique with biochanin A (BCA) as a template, giving narrowly dispersed microspheres with a regular shape. As the functional monomer, (dimethylamino)ethyl methacrylate (-DEM) turned out to be better than methacrylic acid (MAA) to get the best-imprinted effects. The MIPs used as sorbents in the MIPs-HF-SLM-SPE extraction process exhibited excellent binding selectivity for BCA, in comparison to non-imprinted polymers as well as its structural analogs (genistein and daidzein). Finally, the developed method was used to detect BCA in urine. Under optimal extraction conditions, the recovery of BCA in urine samples (using 4.5 mL sample spiked with 10 mu g L-1) was over 41%, with a coefficient of variation (CV) < 6.6% (n = 5). The detection limit (LOD) and quantification limit (LOQ) for BCA analysis in urine were 0.41 and 1.36 mu gL(-1), respectively. (C) 2019 Elsevier B.V. All rights reserved.