ESCHERICHIA-COLI DELTA1-PYRROLINE-5-CARBOXYLATE REDUCTASE - GENE SEQUENCE, PROTEIN OVERPRODUCTION AND PURIFICATION
ESCHERICHIA-COLI DELTA1-PYRROLINE-5-CARBOXYLATE REDUCTASE - GENE SEQUENCE, PROTEIN OVERPRODUCTION AND PURIFICATION
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DOI：
10.1093/nar/10.23.7701
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发表时间：
1982-01-01
影响因子：
14.9
通讯作者：
KRETSCHMER, PJ
中科院分区：
文献类型：
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作者：
DEUTCH, AH;SMITH, CJ;KRETSCHMER, PJ
测定了编码Δ1-吡咯啉-5-羧酸（PCA）还原酶的土方ichiacoliproC基因的序列。通过携带λpL启动子的表达质粒过量表达proC基因产物，使PCA还原酶经亲和吸附层析纯化至均一。纯化的proC蛋白1 s经NH_2和C 00 H-末端分析及氨基酸组成与已报道的基因序列一致。前C单体的分子量为28，112。
The sequence of theEscherichiacoliproCgene which encodes for Δ1-pyrroline-5-carboxylate (PCA) reductase was determined. Overproduction of theproCgene product via an expression plasmid carrying the bacteriophageλpL promoter allowed the purification to homogeneity of PCA reductase by affinity adsorption chromatography. NH2and C00H-term1nal analysis and amino add composition of the purifiedproCprotein 1s consistent with the gene sequence reported. The molecular weight of theproCmonomer is 28,112.