Quantitative stereological evaluation of the gracile and cuneate nuclei and their projection neurons in the rat
Quantitative stereological evaluation of the gracile and cuneate nuclei and their projection neurons in the rat
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DOI：
10.1002/cne.10747
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发表时间：
2003-09-01
影响因子：
2.5
通讯作者：
Avendaño, C
中科院分区：
文献类型：
--
作者：
Bermejo, PE;Jiménez, CE;Avendaño, C
用体视学方法测量了大鼠背柱核（DCN）的体积和神经元数目。这些方法应用于对照动物和接受注射辣根过氧化物酶在丘脑，小脑，或脊髓的情况下，尼氏染色切片。另外一些病例接受了相同结构的荧光示踪剂组合，以检查一些逆行标记的神经元是否向不同的靶点发送了侧支。DCN的平均体积为0.81 mm（3）（范围为0.65-1.10 mm（3）），其中3%、39%和59%分别对应于Bischoff核（Bi）、薄束核（Gr）和楔形核（Cu）。在Cu内，中间分区（CuM）是最大的（42%），其次是吻侧分区（CuR; 36%）和尾侧分区（CuC; 22%）。DCN中神经元的平均总数为16，000（范围12，400 - 19，500），其中2.4%、34.0%和63.6%分别对应于Bi、Gr和Cu。在Cu中，CuM包含所有神经元的48%，27%对应于CuR，25%对应于CuC。整个DCN和Cu的动物间变异性是中等的，但当考虑单个核时会增加。大约80%的DCN神经元投射到丘脑，3%投射到脊髓，7%投射到小脑。丘脑投射细胞在CuM和Gr中较多（83%），而在Bi和CuC中相对较少（72-74%）。向脊髓投射的DCN神经元多出现在CuC和CuM。投射到小脑的神经元中有三分之二位于CuR，20%位于CuM，15%位于GR。一小部分神经元同时投射到脊髓和丘脑。
Stereological methods were employed to estimate the volume and neuron numbers of the rat dorsal column nuclei (DCN). These methods were applied to Nissl-stained sections from control animals and cases that received injections of horseradish peroxidase in the thalamus, the cerebellum, or the spinal cord. Additional cases received combinations of fluorescent tracers in the same structures, to examine whether some of the retrogradely labeled neurons sent collaterals to different targets. The mean volume of the DCN is 0.81 mm(3) (range 0.65-1.10 mm(3)), of which 3%, 39%, and 59% correspond, respectively, to the nucleus of Bischoff (Bi), the gracile (Gr), and the cuneate (Cu) nuclei. Within Cu, the middle division (CuM) is the largest (42%), followed by the rostral (CuR; 36%) and caudal (CuC; 22%) divisions. The mean total number of neurons in the DCN is 16,000 (range 12,400-19,500), of which 2.4%, 34.0% and 63.6% correspond, respectively, to Bi, Gr, and Cu. Within Cu, CuM contains 48% of all neurons, and 27% correspond to CuR and 25% to CuC. Interanimal variability is moderate for the whole DCN and Cu but increases when individual nuclei are considered. About 80% of DCN neurons project to the thalamus, 3% to the spinal cord, and 7% to the cerebellum. Thalamic-projecting cells are more numerous in CuM and Gr (83%), and relatively less common in Bi and CuC (72-74%). Most of the DCN neurons projecting to the spinal cord appear in CuC and CuM. Two-thirds of the neurons projecting to the cerebellum are located in CuR, 20% in CuM, and 15% in Gr. A small fraction of neurons projects simultaneously to spinal cord and thalamus.