Differentiation of murine embryonic stem cells induces progesterone receptor gene expression.
Differentiation of murine embryonic stem cells induces progesterone receptor gene expression.
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小鼠胚胎干细胞的分化诱导孕激素受体基因表达。
DOI：
10.1016/j.yexcr.2005.09.005
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Fritsch,MichaelK
中科院分区：
文献类型：
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作者：
Sauter,CarleyN;McDermid,RebeccaL;Weinberg,AmyL;Greco,TamaraL;Xu,Xiaojie;Murdoch,FernE;Fritsch,MichaelK
类固醇激素受体在早期胚胎发育中的作用仍然未知。显然，器官发生过程中的表达对于组织特异性发育很重要。然而，孕激素受体 (PR) 和雌激素受体 (ERα、ERβ) 在小鼠和其他物种的囊胚阶段的早期发育过程中表达，但对于胚胎活力而言并不是必需的。我们利用小鼠胚胎干（mES）细胞模型来研究这些受体在分化过程中的表达调节。令人惊讶的是，响应观察到的分化信号的基因表达的最早变化之一是 PR 基因诱导。它与模式基因 Hoxb1 和 Hoxa5 的表达时间过程相似。出乎意料的是，PR 基因表达不受内源 ER 或瞬时过表达 ERα 以雌激素依赖性方式调节。我们的结果表明，与成体组织相比，mES 细胞内 PR 基因调控存在潜在的新机制，并且在早期 mES 细胞分化过程中 PR 作用的独特靶点的可能性。
The role of steroid hormone receptors in very early embryonic development remains unknown. Clearly, expression during organogenesis is important for tissue-specific development. However, progesterone receptor (PR) and estrogen receptors (ERα, ERβ) are expressed during early development through the blastocyst stage in mice and other species, and yet are not essential for embryonic viability. We have utilized the mouse embryonic stem (mES) cell model to investigate the regulated expression of these receptors during differentiation. Surprisingly, one of the earliest changes in gene expression in response to a differentiation signal observed is PR gene induction. It parallels the time course of expression for the patterning genes Hoxb1 and Hoxa5. Unexpectedly, PR gene expression is not regulated in an estrogen-dependent manner by endogenous ERs or by transiently overexpressed ERα. Our results suggest a potentially novel mechanism of PR gene regulation within mES cells compared to adult tissues and the possibility of unique targets of PR action during early mES cell differentiation.