ANALYSIS OF SERUM INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS USING WESTERN BLOTTING - USE OF THE METHOD FOR TITRATION OF THE BINDING-PROTEINS AND COMPETITIVE-BINDING STUDIES
ANALYSIS OF SERUM INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEINS USING WESTERN BLOTTING - USE OF THE METHOD FOR TITRATION OF THE BINDING-PROTEINS AND COMPETITIVE-BINDING STUDIES
复制标题
DOI：
10.1016/0003-2697(86)90507-5
复制
发表时间：
1986-04-01
影响因子：
2.9
通讯作者：
BINOUX, M
中科院分区：
文献类型：
--
作者：
HOSSENLOPP, P;SEURIN, D;BINOUX, M
采用硝酸纤维素凝胶转移技术对人血清中胰岛素样生长因子（IGF）结合蛋白进行了研究。正常和垂体后垂体血清经十二烷基硫酸钠-聚丙烯酰胺凝胶电泳，然后电印迹到硝化纤维或尼龙膜。需要Nonidet-P40（3%）和Tween 20（0.1%）进行淬火，并允许通过125i标记的IGFI或IGF II的放射自显影覆盖来检测IGF结合蛋白。几种形式的IGF结合蛋白已被鉴定为分子量为41,500,38,500,34,000,30,000和24,000。对转移的IGF结合蛋白进行了滴定和与IGF的竞争结合研究，表明通过该技术分离的结合蛋白可以被表征。
A nitrocellulose gel transfer technique has been adapted to study the insulin-like growth factor (IGF) binding proteins of human serum. Normal and hypopituitary sera were subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by electroblotting to nitrocellulose or nylon membrane. Nonidet-P40 (3%) and Tween 20 (0.1%) were required for quenching and to allow detection of the IGF binding proteins by autoradiography overlay with either 125I-labeled IGFI or IGF II. Several forms of IGF binding protein have been identified with molecular weights of 41,500, 38,500, 34,000, 30,000, and 24,000. Titration and competitive binding studies with IGF were performed on the transferred IGF binding proteins, indicating that binding proteins isolated by this technique can be characterized.