Expression profile of WNT, FZD and sFRP genes in human hematopoietic cells
Expression profile of WNT, FZD and sFRP genes in human hematopoietic cells
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DOI：
10.1016/j.leukres.2010.02.009
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发表时间：
2010-07-01
影响因子：
2.7
通讯作者：
Sercan, Hakki Ogun
中科院分区：
文献类型：
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作者：
Sercan, Zeynep;Pehlivan, Melek;Sercan, Hakki Ogun
鉴定白血病细胞特异性Wnt信号通路中的基因表达差异可能会受到缺乏关于正常血细胞中WNT基因表达的验证知识的阻碍。在这项研究中，我们的目的是确定人WNT，FZD和sFRP基因在正常成人骨髓，T和B细胞的表达谱;沿着造血细胞系K562，HL 60，Jurkat和Namalwa。对16名供体的骨髓和外周血进行了评估，并将我们的结果与GeneNote数据库表达阵列进行了比较。在骨髓中，仅WNT 3、WNT 10A、FZD 3、FZD 7和sFRP 1基因组成型表达。除了上述基因之外，淋巴细胞还表达WNT 7A、WNT 9 B、FZD 6和FZD 7，但T细胞的不同之处在于它们失去sFRP 1表达并获得WNT 16的组成型表达。在各种血细胞中Wnt基因的“正常”分布将为研究白血病转化过程中的造血和细胞过程提供基础。(C)2010爱思唯尔有限公司保留所有权利。
Identifying gene expression differences in the Wnt signaling pathway specific to leukemic cells can be hampered by the lack of verified knowledge on the expression of WNT genes in normal blood cells. In this study we aimed to determine the expression profile of human WNT, FZD and sFRP genes in normal adult bone marrow, T and B cells; along with the hematopoietic cell lines K562, HL60, Jurkat and Namalwa. Bone marrow and peripheral blood from 16 donors were evaluated and our results were compared with the GeneNote database expression arrays. In bone marrow, only WNT3, WNT10A, FZD3, FZD7 and sFRP1 genes are constitutively expressed. Lymphocytes express WNT7A, WNT9B, FZD6 and FZD7 in addition to the genes above, but T cells differ in that they lose sFRP1 expression and gain constitutive expression of WNT16. An established "normal" profile of the Wnt genes in various blood cells will provide a fundamental basis for research investigating hematopoiesis and cellular processes during leukemic transformation. (C) 2010 Elsevier Ltd. All rights reserved.