Stimulation of human mesenchymal stem cells and osteoblasts activities in vitro on silicon-releasable scaffolds
Stimulation of human mesenchymal stem cells and osteoblasts activities in vitro on silicon-releasable scaffolds
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DOI：
10.1002/jbm.a.32181
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发表时间：
2009-10-01
影响因子：
4.9
通讯作者：
Kasuga, Toshihiro
中科院分区：
文献类型：
--
作者：
Obata, Akiko;Kasuga, Toshihiro
在含有或不含有机因子（如地塞米松（Dex）和β-甘油磷酸盐（β-GP））的培养基中，评估了硅可释放支架、掺杂正戊烷的聚（乳酸）和涂覆有羟基碳酸盐磷灰石（SPV-H）的球霰石复合物上的人成骨细胞（HOBS）和间充质干细胞（MSC）的细胞活性，以诱导矿化或分化。作为对照，使用聚（乳酸）和球霰石制备不含硅的复合膜（用PV-H表示）。在SPV-H上培养的HOBS在不含有机因子的培养基中培养21天后形成了一些聚集体，即骨结节，而在PV-H上没有观察到聚集体。激光拉曼光谱表明磷酸钙在SPV-H上以HOB形式沉淀。SPV-H中的硅物种促进了HOBS的矿化。使用MSC的培养测试显示，在SPV-H上培养的细胞中的碱性磷酸酶（ALP）活性水平在不含Dex和β-GP的培养基中培养21天期间增加。在PV-H上培养的MSC中水平不变。在培养基中添加Dex和beta-GP的情况下，在SPV-H上培养的MSC的ALP活性水平在21天的培养期间的所有时间点均高于PV-H。SPV-H中的硅物质被认为是诱导和增强MSC的成骨分化。(C)2008 Wiley Periodicals，Inc. J Biomed Mater Res 91A：11-17，2009
Cellular activities of human osteoblasts (HOBS) and mesenchymal stem cells (MSCs) on a silicon-releasable scaffold, siloxane-doped poly(lactic acid) and vaterite composite coated with hydroxycarbonate apatite (SPV-H), were estimated using a medium with or without organic factors, such as dexamethasone (Dex) and beta-glycerophosphate (beta-GP), for inducing mineralization or differentiation. As a control, a composite film containing no silicon (denoted by PV-H) was prepared using poly(lactic acid) and vaterite. HOBS cultured on SPV-H formed some agglomerates, bone nodules, after a 21-day culture in a medium without the organic factors, whereas no agglomerate was observed on PV-H. Laser Raman spectra implied that calcium phosphate precipitated in HOBs on the SPV-H. The silicon species in SPV-H stimulated HOBS to mineralization. The culture tests using MSCs show that the level of alkaline phosphatase (ALP) activity in the cells cultured on SPV-H increased during the 21-day culture in a medium without Dex and beta-GP. The level was unchanged in MSCs cultured on PV-H. In the case of supplementing Dex and beta-GP to the medium, the level of ALP activity in MSCs cultured on SPV-H was higher than that on PV-H at all time points during the 21-day culture. The silicon species in SPV-H were regarded to induce and enhance the osteogenic differentiation of MSCs. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91A: 11-17, 2009