MOTILITY AND MULTIPLICATION OF MYCOPLASMA PNEUMONIAE - A PHASE CONTRAST STUDY
MOTILITY AND MULTIPLICATION OF MYCOPLASMA PNEUMONIAE - A PHASE CONTRAST STUDY
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DOI：
10.1159/000162074
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发表时间：
1968-01-01
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影响因子：
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通讯作者：
BREDT, W
中科院分区：
文献类型：
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作者：
BREDT, W
讨论支原体的增殖一直是一个有争议的问题[5, 8]。最近的电子显微镜结果显示了小型支原体和山羊支原体菌株二元裂变的证据[10]。 Furness等人解释了过滤和紫外线处理的培养物的菌落计数。|(»|假设肺炎支原体是二元裂变。但到目前为止，还没有关于在流体环境中连续观察支原体存活和繁殖的报道。此处描述的对肺炎支原体的观察结果表明二元裂变（或分裂）是正常的繁殖模式。此外，它们表明复制时间约为3小时，比之前计算的时间短得多。小时 [12] 或 6.5 小时 [6]。此处使用的介质以外的介质可能会导致更短的时间。这些显微镜结果与菌落计数获得的结果之间的差异可能是由于肉汤中“球粒”[9]、“簇”[6] 或“菌落”] 2] 的形成造成的。这些结构阻碍了通过菌落计数对肉汤中单一生物体的真实数量进行精确评估。在没有任何可见隔膜的情况下的分离模式，以及在不损失细胞材料的情况下破坏连接线的模式，尚无法解释。从初步的电子显微镜研究（Limit 和 Grimley，未发表的结果）看来，在“成熟”丝中，细胞内容物可能集中在旋钮内。
DiscussionThe multiplication of mycoplasmas has been a matter of con troversy [5, 8]. Recent electron microscopic results have shown evidence for binary fission in Mycoplasma ho minis and a caprine mycoplasma strain [10]. Interpreting colony counts of filtered and UV-treated cultures, Furness et al.|(»| postulate a binary fission for M. pneumoniae. Rut so far there are no reports of continuous observations of living and multiplying mycoplasmas in a fluid environment. The results of such observations on M. pneumoniae as described here show binary fission (or division) to be the nor mal mode of multiplication. Furthermore they indicate a replica tion time of about 3 h, a time much shorter than the previously calculated It) h [12] or 6.5 h [6]. Media other than those used here might result in still shorter periods. The differences between these microscopic results and those obtained by colony counting could be due to the formation of ‘spherules’[9],‘clusters'[6] or ‘colonies’] 2] in the broth. These structures are preventing an exact evalua tion by colony counting of the true number of single organisms in broth.The mode of separation in the absence of any visible septa, and the disruption of the connecting string without loss of cellular material, cannot yet be explained. From preliminary electron microscopic studies (limit and Grimley, unpublished results) it seems possible that in'mature'filaments the cell content is con centrated within the knobs.