Toxicity and histopathology of the growth regulator pyriproxyfen to adults and eggs of the cat flea (Siphonaptera: Pulicidae)
Toxicity and histopathology of the growth regulator pyriproxyfen to adults and eggs of the cat flea (Siphonaptera: Pulicidae)
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DOI：
10.1093/jmedent/33.4.670
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发表时间：
1996-07-01
影响因子：
2.1
通讯作者：
Meola, S
中科院分区：
文献类型：
--
作者：
Meola, R;Pullen, S;Meola, S
成年猫蚤，Ctenocephalidesfels (Bouche)，连续暴露于吡丙醚，在 8-10 天内死亡。 7日龄成虫的显微镜检查表明死亡是由脂肪体、马氏小管、中肠上皮、唾液腺细胞和其他内部组织的组织病理学损伤引起的。无论将未喂食的成虫放在经过处理的填充纸上还是将成虫喂食到经过处理的狗毛上，跳蚤都会被杀死。除了对成人的这些毒性作用外，吡丙醚还诱导成熟卵母细胞中大自噬空泡的形成，导致卵黄部分重吸收、细胞核变性和卵泡上皮溶解。因此，经过处理的跳蚤产下的大多数卵都缺乏细胞核，绒毛膜形成不良，并且在排卵期间破裂。这些实验室结果表明，将跳蚤持续暴露在宿主动物身上的吡丙醚可以防止活卵沉积并最终杀死成虫，从而控制跳蚤发育的所有阶段。
Adult cat fleas, Ctenocephalides felts (Bouche), exposed continuously to pyriproxyfen died within 8-10 d. Microscopic examination of 7-d-old adults indicated death was caused by histopathological damage to fat body, Malpighian tubules, midgut epithelia, salivary gland cells, and other internal tissues. Fleas were killed pyriproxyfen regardless of whether by they were held as unfed adults on treated filler paper or as feeding adults on treated dog hair. In addition to these toxic effects on adults, pyriproxyfen also induced formation of large autophagic vacuoles in maturing oocytes leading to partial reabsorption of yolk, degeneration of the nucleus, and lysis of the follicular epithelium. Consequently, most of the eggs laid by treated fleas lacked a nucleus, had a poorly formed chorion, and were ruptured during ovulation. These laboratory results suggest that continuous exposure of fleas to pyriproxyfen on a host animal could prevent deposition of viable eggs and eventually kill adults, thereby controlling ail stages of flea development.