Adsorbent-Based Downstream-Processing of the Decarboxylase-Based Synthesis of 2,6-Dihydroxy-4-methylbenzoic Acid
Adsorbent-Based Downstream-Processing of the Decarboxylase-Based Synthesis of 2,6-Dihydroxy-4-methylbenzoic Acid
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DOI：
10.1021/acs.oprd.8b00104
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发表时间：
2018-08-01
影响因子：
3.4
通讯作者：
von Langermann, Jan
中科院分区：
文献类型：
--
作者：
Meyer, Lars-Erik;Plasch, Katharina;von Langermann, Jan
在本案例研究中，研究了使用来自Aspergilluslaughus的非氧化性2，3-二羟基苯甲酸脱羧酶（2，3-DHBD_Ao），随后通过基于吸附剂的下游方法的3，5-二羟基甲苯（苔黑酚）的区域选择性酶促羧化。产物2，6-二羟基-4-甲基苯甲酸（DHMBA）在本文中通过吸附-解吸循环纯化，并且随后获得纯度>99%，而没有从其反应溶液中完全消除过量的碳酸氢盐。研究了十种吸附树脂从反应溶液中回收产物的能力，而氯化物形式的强阴离子交换树脂Dowex 1x 2即使在碳酸氢盐浓度>3 mol.L-1时，亲和力也> 99%。用2 mol.L ~（-1）HCl/丙酮混合溶液解吸树脂，丙酮蒸发结晶。该提出的概念不需要最终的柱制备步骤，并且提高了生物催化反应系统的总体原子效率。
In this case study the regioselective enzymatic carboxylation of 3,5-dihydroxytoluene (orcinol) using the nonoxidative 2,3-dihydroxybenzoic acid decarboxylase from Aspergillus oryzae (2,3-DHBD_Ao), followed by an adsorbent based downstream approach, has been investigated. The product 2,6-dihydroxy-4-methylbenzoic acid (DHMBA) was herein purified by an adsorption-desorption cycle and subsequently obtained with purities >99% without a full elimination of the excess bicarbonate from its reaction solution. Ten adsorbent resins were studied in respect of their ability to recover the product from the reaction solution, whereas the strong anion exchange resin Dowex 1x2 in its chloride form showed affinities >99%, even at bicarbonate concentrations of >3 mol.L-1. Desorption from loaded resin was carried out by a 2 mol.L-1 HCl/acetone mixture, followed by product crystallization during acetone evaporation. This presented concept does not require a final column preparation step and improves the overall atom efficiency of the biocatalytic reaction system.