The clinical, biochemical, haematological and pathological effects of long-term administration of Ipomoea carnea to growing goats
The clinical, biochemical, haematological and pathological effects of long-term administration of Ipomoea carnea to growing goats
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DOI：
10.1023/a:1024036225641
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发表时间：
2003-05-01
影响因子：
2.2
通讯作者：
Spinosa, HS
中科院分区：
文献类型：
--
作者：
Schumaher-Henrique, B;Górniak, SL;Spinosa, HS
Ipomoea carnea已被认为是几起中毒事件的责任，主要是在山羊中。这种植物含有苦马豆素，抑制酸或溶酶体α-甘露糖苷酶，导致细胞空泡化。本研究的目的是评估I。当四种不同剂量的这种植物喂给生长中的山羊时，将25只公山羊分为5组，1个对照组和4个实验组，每天每公斤活重接受2.5，5.0，10.0和30.0 g的植物，持续4个月。采集血样进行血液学和生化测定，并采集部分组织碎片进行组织病理学研究。所有实验山羊在整个试验期间摄入该植物，表现出眼球震颤、肌肉震颤、后肢无力和共济失调。与对照组的山羊相比，从实验的第六周开始，它们的丙氨酸氨基转移酶（ALT）也显著增加。用I处理的山羊血红蛋白浓度显著降低。carnea。组织病理学显示，在接受该植物的动物中，肝脏、胰腺、甲状腺和肾脏细胞以及中枢神经系统的神经元中出现了退行性空泡改变。所有这些变化均以剂量依赖性方式发生。
Ipomoea carnea has been held responsible for several poisoning episodes, mainly in goats. This plant contains swainsonine, which inhibits acid or lysosomal alpha-mannosidase enzyme, causing cellular vacuolization. The objective of this study was to evaluate I. carnea toxicosis when four different doses of this plant were fed to growing goats. Twenty-five male goats were divided into five groups, one control group and four experimental groups that received 2.5, 5.0, 10.0 and 30.0 g of the plant per kg of live weight per day for 4 months. Blood samples were collected for haematological and biochemical determinations and fragments from some tissues were collected for histopathological study. All the experimental goats ingested the plant throughout the trial, presenting nystagmus, muscle tremors, weakness of the hind limbs and ataxia. They also had a significant increase in alanine aminotransferase (ALT) from the sixth week of the experiment compared to the goats in the control group. There was a significant reduction in haemoglobin concentration in the goats treated with I. carnea. Histopathology revealed degenerative vacuolar alteration in the liver, pancreas, thyroid and kidney cells, and in the neurons of the central nervous system in the animals that received the plant. All these alterations occurred in a dose-dependent manner.