Mono- and dihydroxyeicosatetraenoic acids alter calcium homeostasis in rabbit neutrophils.
Mono- and dihydroxyeicosatetraenoic acids alter calcium homeostasis in rabbit neutrophils.
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单羟基二十碳四烯酸和二羟基二十碳四烯酸改变兔中性粒细胞的钙稳态。
DOI：
10.1172/jci110191
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Goetzl,EJ
中科院分区：
文献类型：
--
作者：
Naccache,PH;Sha'afi,RI;Borgeat,P;Goetzl,EJ
体外研究了花生四烯酸脂氧合产物表达中性粒细胞趋化活性对兔腹膜中性粒细胞对45Ca摄取的影响。在最佳趋化浓度下，5-和11-氢过氧二十碳四烯酸、11-和12l -羟基二十碳四烯酸和白三烯B4在1分钟内增加了对照中性粒细胞对45Ca的吸收，平均提高了212-694%，而5(S)-羟基二十碳四烯酸的吸收率为75%，而15-氢过氧二十碳四烯酸和15-羟基二十碳四烯酸的吸收率则为75%。刺激45Ca吸收的因子的效价大致与趋化性相似。白三烯B4除了刺激兔中性粒细胞吸收45Ca的速率外，还取代了细胞内的钙。白三烯b4诱导的钙稳态变化的最终结果是短暂地提高细胞内可交换钙的水平。内源性花生四烯酸的中性粒细胞脂氧合酶代谢物迅速增强45Ca的流入，这支持了这种代谢物，特别是白三烯B4在中性粒细胞和其他激素敏感细胞中调控细胞内游离钙水平的可能作用，其中这种阳离子是第二信使。
Products of the lipoxygenation of arachidonic acid that express neutrophil chemotactic activity were examined in vitro for effects on the uptake of 45Ca by rabbit peritoneal neutrophils. At optimally chemotactic concentrations, 5- and 11-hydroperoxyeicosatetraenoic acid, 11- and 12L-hydroxyeicosatetraenoic acid, and leukotriene B4 enhanced 45Ca uptake within 1 min by a mean of 212-694% of the values for control neutrophils incubated in buffer alone, as compared with 75% for 5(S)-hydroxyeicosatetraenoic acid and no effect for 15-hydroperoxyeicosatetraenoic acid and 15-hydroxyeicosatetraenoic acid. The approximate rank of potency of the factors stimulating 45Ca uptake was similar to that observed for chemotaxis. Leukotriene B4, in addition to stimulating the rate of 45Ca uptake into rabbit neutrophils, also displaced intracellular calcium. The net result of the leukotriene B4-induced changes in calcium homeostasis ws to elevate transiently the intracellular level of exchangeable calcium. That neutrophil lipoxygenase metabolites of endogenous arachidonic acid rapidly enhance the influx of 45Ca supports a possible role for such metabolites in general, and leukotriene B4 in particular, in the regulation of the intracellular levels of free calcium in the neutrophils and possibly in other hormonally sensitive cells in which this cation is a second messenger.