Extensive junctional diversity of rearranged human T cell receptor delta genes.
Extensive junctional diversity of rearranged human T cell receptor delta genes.
复制标题
重排的人类 T 细胞受体 delta 基因的广泛连接多样性。
DOI：
10.1126/science.3259726
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Krangel,MS
中科院分区：
文献类型：
--
作者：
Hata,S;Satyanarayana,K;Devlin,P;Band,H;McLean,J;Strominger,JL;Brenner,MB;Krangel,MS
人T细胞受体δ（TCR δ）基因编码外周血和胸腺T细胞亚群上发现的TCR γδ-CD 3复合物的一种组分。通过表征TCR γδ细胞系中的重排并确定这些细胞系中代表功能性和非功能性转录物的互补DNA克隆的结构来估计人类TCR δ多样性。在所有功能性转录物中鉴定出一个Vδ片段和一个Jδ片段，尽管在截短的转录物中鉴定出一个独特的Jδ片段。此外，还鉴定出一个Dδ元素，并获得了使用另一个Dδ元素的证据。因此，人类TCR δ基因似乎使用有限数量的生殖系元件。然而，明显使用两个Dδ元件串联，加上不精确的连接和N核苷酸的大量掺入，在连接区产生了前所未有的变异性。
The human T cell receptor δ (TCR δ) gene encodes one component of the TCR γδ-CD3 complex found on subsets of peripheral blood and thymic T cells. Human TCR δ diversity was estimated by characterizing rearrangements in TCR γδ cell lines and determining the structures of complementary DNA clones representing functional and nonfunctional transcripts in these cell lines. One Vδ segment and one Jδ segment were identified in all functional transcripts, although a distinct Jδ segment was identified in a truncated transcript. Further, one Dδ element was identified, and evidence for the use of an additional Dδ element was obtained. Thus human TCR δ genes appear to use a limited number of germline elements. However, the apparent use of two Dδ elements in tandem coupled with imprecise joining and extensive incorporation of N nucleotides generates unprecedented variability in the junctional region.