Endogenous steroid levels in the human prostate from birth to old age: a comparison of normal and diseased tissues.
Endogenous steroid levels in the human prostate from birth to old age: a comparison of normal and diseased tissues.
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DOI：
10.1677/joe.0.0780007
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发表时间：
1978-07
期刊：
影响因子：
--
通讯作者：
G. Hammond
中科院分区：
文献类型：
--
作者：
G. Hammond
溶剂提取单个（300--600 mg湿组织）样品并在羟基烷氧基丙基葡聚糖微柱上对提取物进行色谱分析后，通过放射免疫测定法测定前列腺组织中的类固醇水平。在正常成人前列腺 (n = 18) 中，类固醇浓度（平均值 +/- S.E.M. ng/g 湿组织）为： 睾酮，0.25 +/- 0.04；雄烯二酮，0.13+/-0.03； 5α-雄甾烷-3,17-二酮（5α-雄甾烷二酮），1.31+/-0.30； 17β-羟基-5α-雄激素-3-酮（5α-二氢睾酮，DHT），1.22 +/- 0.14； 5α-雄甾烷-3α，17β-二醇（3α-雄甾烷二醇），4.32+/-0.49；雄酮，4.15 +/- 1.07；黄体酮，0.39+/-0.07； 17α-羟基孕酮，0.42 +/- 0.06。来自外腺区域的类固醇组织的浓度在尿道周围区域中发现的范围内。在新生男孩的前列腺中，所有类固醇的浓度都很高，包括黄体酮和 17α-羟基黄体酮。除了3α-雄甾烷二醇（其水平在此年龄后趋于增加）之外，所有类固醇的浓度在婴儿和青春期前列腺中均较低，但大多数类固醇在成人组织中以不同程度重新建立。这对于 5α-雄甾烷二酮和雄酮尤其明显，并且表明这两种雄激素可能在成熟前列腺中具有功能性作用。 10 名良性前列腺肥大 (BPH) 患者的组织中睾酮、雄烯二酮、5α-雄烯二酮、孕酮和 17α-羟基孕酮的浓度与正常成人组织中的浓度相似。与正常组织相比，腺瘤中DHT浓度显着升高（5.33+/-0.46ng/g），3α-雄甾烷二醇和雄酮水平降低（分别为1.40+/-0.12ng/g）。各种雄激素的浓度显示出显着的相互关系，这是正常组织和良性前列腺增生组织的特征。在所研究的未经治疗的前列腺癌组织样本中，睾酮、雄烯二酮、DHT和3α-雄烷二醇的浓度特别高，而5α-雄烷二酮和雄甾酮的浓度非常低。雌激素治疗后，睾酮和雄烯二酮实际上消失了，而其代谢物的浓度虽降低但显着，但其比例仍与未治疗的癌组织中观察到的比例相似。在治疗条件下，雌激素被认为可以影响雄激素的摄取。
The levels of steroids in prostatic tissues were determined by radioimmunoassay after solvent extraction of a single (300--600 mg wet tissue) sample and chromatography of extracts on hydroxyalkoxypropyl Sephandex microcolumns. In normal adult prostates (n = 18) the concentrations of steroids (mean +/- S.E.M. ng/g wet tissue) were: testosterone, 0.25 +/- 0.04; androstenedione, 0.13 +/- 0.03; 5alpha-androstane-3,17-dione (5alpha-androstanedione), 1.31 +/- 0.30; 17beta-hydroxy-5alpha-androstan-3-one (5alpha-dihydrotestosterone, DHT), 1.22 +/- 0.14; 5alpha-androstane-3alpha, 17 beta-diol (3alpha-androstanediol), 4.32 +/- 0.49; androsterone, 4.15 +/- 1.07; progestrone, 0.39 +/- 0.07; 17alpha-hydroxyprogestrone, 0.42 +/- 0.06. Concentrations of of steroids tissues from outer-gland regions were within the ranges found in the periurethral regions. In the prostates of newborn boys, the concentrations of all steroids were high, including progesterone and 17alpha-hydroxyprogesterone. Apart from 3alpha-androstanediol, the levels of which tend to increase after this age, the concentrations of all steroids were lower in the infant and pubertal prostates, but most were re-established to a variable extend in the adult tissues. This was particularly evident with respect to 5alpha-androstanedione and androsterone, and it is suggested that these two androgens may have a functional role in the mature prostate. The concentrations of testosterone, androstenedione, 5alpha-androstanedione, progesterone and 17alpha-hydroxyprogesterone in tissue taken from ten patients with benign prostatic hypertrophy (BPH) were similar to those in normal adult adult tissue. The concentration of DHT was markedly raised (5.33 +/- 0.46 ng/g) and the level of 3alpha-androstanediol and androsterone were reduced (1.40 +/- 0.12 ng/g respectively) in adenomatous compared with normal tissue. The concentrations of various androgens displayed remarkable interelationships which characterize normal and BPH tissues. The concentrations of testosterone, androstenedione, DHT and 3alpha-androstanediol were particularly high in the untreated prostatic carcinomatous tissue sample investigated, whereas the concentrations of 5alpha-androstanedione and androsterone were very low. After oestrogen treatment, testosterone and androstenedione virtually disappeared, while reduced but significant concentrations of their metabolites remained in similar proportions to those observed in the untreated carcinomatous tissue. Under the conditions of therapy, oestrogens are suggested to influence the uptake of androgens.