Parametric analyses of anxiety in zebrafish scototaxis
Parametric analyses of anxiety in zebrafish scototaxis
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DOI：
10.1016/j.bbr.2010.01.031
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发表时间：
2010-06-26
影响因子：
2.7
通讯作者：
Gouveia, Amauri, Jr.
中科院分区：
文献类型：
--
作者：
Maximino, Caio;de Brito, Thiago Marques;Gouveia, Amauri, Jr.
趋暗性，即偏爱黑暗环境而不利于明亮环境，是斑马鱼焦虑的一个指标。在这项工作中，我们通过分析四个实验中探索行为的时空模式（在设备的白色隔间中花费的时间和隔间之间的穿梭频率）和游泳行为图（在白色隔间中的趋触性、冻结和突发游泳）来分析对白色隔间的回避。在实验 1 中，我们证明了白色回避和运动的时空测量在单个 15 分钟的过程中不会形成习惯。在实验 2 和 3 中，我们证明，无论试验间隔是 15 分钟还是 24 小时，运动活动都会习惯于重复暴露于该装置；然而，在这两个实验中都没有观察到回避白色的习惯。在实验 4 中，我们将动物限制在白色隔间中 3 个 15 分钟的时段，然后在标准偏好测试中记录时空和行为图测量。在这些强制暴露之后，白色回避和运动活动与非封闭动物相比没有表现出差异，但在白色隔间中的突发游泳、触动性和冻结都减少了。这些结果表明，回避白色区室或接近黑色区室都不能解释斑马鱼在趋光性测试中的行为。 (C) 2010 Elsevier B.V. 保留所有权利。
Scototaxis, the preference for dark environments in detriment of bright ones, is an index of anxiety in zebrafish. In this work, we analyzed avoidance of the white compartment by analysis of the spatiotemporal pattern of exploratory behavior (time spent in the white compartment of the apparatus and shuttle frequency between compartments) and swimming ethogram (thigmotaxis, freezing and burst swimming in the white compartment) in four experiments. In Experiment 1, we demonstrate that spatiotemporal measures of white avoidance and locomotion do not habituate during a single 15-min session. In Experiments 2 and 3, we demonstrate that locomotor activity habituates to repeated exposures to the apparatus, regardless of whether inter-trial interval is 15-min or 24-h; however, no habituation of white avoidance was observed in either experiment. In Experiment 4, we confined animals for three 15-min sessions in the white compartment prior to recording spatiotemporal and ethogram measures in a standard preference test. After these forced exposures, white avoidance and locomotor activity showed no differences in relation to non-confined animals, but burst swimming, thigmotaxis and freezing in the white compartment were all decreased. These results suggest that neither avoidance of the white compartment nor approach to the black compartment account for the behavior of zebrafish in the scototaxis test. (C) 2010 Elsevier B.V. All rights reserved.