Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
Off-resonance plasmonic enhanced femtosecond laser optoporation and transfection of cancer cells
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DOI：
10.1016/j.biomaterials.2011.11.062
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发表时间：
2012-03-01
期刊：
影响因子：
14
通讯作者：
Meunier, Michel
中科院分区：
文献类型：
--
作者：
Baumgart, Judith;Humbert, Laure;Meunier, Michel
描述了使用非共振等离子体金纳米颗粒的基于飞秒激光的转染方法。对于人类癌症黑色素瘤细胞，该处理导致70%的非常高的穿孔率，转染效率比常规脂质转染高三倍，并且毒性非常低（< 1%）。非共振激光激发抑制了纳米颗粒断裂成可能有毒的DNA嵌入颗粒。这种高效低毒的方法是一种有前途的替代病毒转染治疗皮肤癌。(C)2011爱思唯尔有限公司保留所有权利。
A femtosecond laser based transfection method using off-resonance plasmonic gold nanoparticles is described. For human cancer melanoma cells, the treatment leads to a very high perforation rate of 70%, transfection efficiency three times higher than for conventional lipofection, and very low toxicity (< 1%). Off-resonance laser excitation inhibited the fracture of the nanoparticles into possibly toxic DNA intercalating particles. This efficient and low toxicity method is a promising alternative to viral transfection for skin cancer treatment. (C) 2011 Elsevier Ltd. All rights reserved.