A Tie2-driven BAC-TRAP transgenic line for in vivo endothelial gene profiling.
A Tie2-driven BAC-TRAP transgenic line for in vivo endothelial gene profiling.
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DOI：
10.1002/dvg.22923
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发表时间：
2016-03
期刊：
影响因子：
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通讯作者：
Huang Z
中科院分区：
文献类型：
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作者：
Santhosh D;Huang Z
最近的技术创新，包括细菌人工染色体为基础的翻译核糖体亲和纯化（BAC-TRAP），极大地促进了细胞类型特异性基因表达的分析在体内，特别是在神经系统。为了更好地研究体内内皮基因表达，我们已经产生了BAC-TRAP转基因小鼠系，其中L10 a核糖体亚基用EGFP标记并置于内皮特异性Tie 2（Tek）启动子的控制下。我们表明，在这条线的转基因表达是广泛的，但具体地说，检测在内皮细胞在几个大脑区域在整个产前和产后的发展，以及在其他器官。我们还表明，这条线的结果在不同阶段的脑组织内皮特异性mRNA的高度显着富集。因此，该BAC-TRAP系为在各种发育、生理和病理条件下进行体内内皮基因谱分析提供了有用的遗传工具。
Recent technological innovations including bacterial artificial chromosome-based translating ribosome affinity purification (BAC-TRAP) have greatly facilitated analysis of cell type-specific gene expression in vivo, especially in the nervous system. To better study endothelial gene expression in vivo, we have generated a BAC-TRAP transgenic mouse line where the L10a ribosomal subunit is tagged with EGFP and placed under the control of the endothelium-specific Tie2 (Tek) promoter. We show that transgene expression in this line is widely, but specifically, detected in endothelial cells in several brain regions throughout pre- and postnatal development, as well as in other organs. We also show that this line results in highly significant enrichment of endothelium-specific mRNAs from brain tissues at different stages. This BAC-TRAP line therefore provides a useful genetic tool for in vivo endothelial gene profiling under various developmental, physiological, and pathological conditions.