Interaction of transducin-α with LGN, a G-protein modulator expressed in photoreceptor cells
Interaction of transducin-α with LGN, a G-protein modulator expressed in photoreceptor cells
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DOI：
10.1016/j.mcn.2004.10.010
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发表时间：
2005-03-01
影响因子：
3.5
通讯作者：
Artemyev, NO
中科院分区：
文献类型：
--
作者：
Kerov, VS;Natochin, M;Artemyev, NO
LGN和G蛋白信号传导激活剂3（AGS3）属于含有G蛋白调节基序（GPR蛋白）的G蛋白调节剂类别。这些分子功能的证据才刚刚开始出现。小鼠视网膜横截面的免疫染色和视网膜的连续切向切片结合免疫印迹分析显示，LGN在感光细胞的内节中表达。双重免疫标记表明，光依赖性易位从外段，α亚基的视觉G蛋白转导（GT α）与LGN共定位在基底部分的内段。LGN和Gt α从视网膜提取物中共沉淀，支持蛋白质之间相互作用的概念。此外，LGN的GPR结构域有效地抑制受体介导的鸟嘌呤核苷酸交换和transducin的稳态GTP酶活性。与Gt α的定位和相互作用表明LGN在调节转导素易位和其他感光细胞功能中的作用。(c)2004爱思唯尔公司All rights reserved.
LGN and activator of G-protein signaling 3 (AGS3) belong to the class of G-protein modulators containing G-protein regulatory motifs (GPR proteins). Evidence for the functions of these molecules has only started to emerge. Immunostaining of mouse retina cross-sections and serial tangential sectioning of the retina combined with immunoblot analysis revealed that LGN is expressed in the inner segments of photoreceptor cells. Double immunolabeling demonstrated that, following light-dependent translocation from the outer segments, the alpha-subunit of the visual G-protein transducin (Gt alpha) colocalizes with LGN in the basal part of the inner segments. LGN and Gt alpha coprecipitate from the retinal extracts, supporting the notion of the interaction between the proteins. Furthermore, the GPR domain of LGN potently inhibits receptor-mediated guanine nucleotide exchange and steady-state GTPase activity of transducin. The localization and interaction with Gt alpha suggest LGN roles in modulation of transducin translocation and other photoreceptor cell functions. (c) 2004 Elsevier Inc. All rights reserved.