Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants
Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants
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DOI：
10.1007/s10681-014-1159-4
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发表时间：
2014
期刊：
影响因子：
1.9
通讯作者：
Tomomi Miyashita;Y. Hoshino
中科院分区：
文献类型：
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作者：
Tomomi Miyashita;Y. Hoshino
我们通过进行野生种质的倍体间和倍体内杂交产生了多倍体Haskap（蓝萼藓）植物。在四倍体（4x）×二倍体（2x）杂交中的胚拯救产生了三倍体植株;相反的2x× 4x杂交未能产生有活力的种子。4x× 4x的倍性内杂交大多产生四倍体，但也有一些六倍体（6x）和八倍体（8x）植株。使用从该杂交获得的六倍体，我们检查了正反4x-6x杂交，发现两者都产生五倍体植物。通过将秋水仙素应用于四倍体产生四倍体;使用该植物进行4x× 8x杂交并辅以胚拯救培养产生三个六倍体植物，具有非整倍体数目的染色体。本研究中获得的几种植物开花并结果。我们讨论了在倍体间和倍体内杂交中产生多倍体植株的总体效率。
We produced polyploid Haskap (Lonicera caeruleavar.emphyllocalyx) plants by performing interploid and intraploid crosses of wild accessions. Embryo rescue in a tetraploid (4x) × diploid (2x) cross produced triploid plants; reciprocal 2x× 4xcross failed to produce viable seeds. Intraploidy crosses of 4x× 4xproduced mostly tetraploids but also several hexaploid (6x) and octoploid (8x) plants. Using hexaploids obtained from this cross, we examined reciprocal 4x–6xcrosses and found that both produced pentaploid plants. An octoploid was produced by applying colchicine to a tetraploid; a 4x× 8xcross using this plant and aided by embryo rescue culture produced three hexaploid plants, with an aneuploid number of chromosomes. Several plants obtained in this study flowered and set fruits. We discuss the overall efficiency of producing polyploid plants in interploid and intraploid crosses.