Quantitative effects of magnesium chloride stress on aggregation of Sup35p in [psi-] yeast cells.
Quantitative effects of magnesium chloride stress on aggregation of Sup35p in [psi-] yeast cells.
复制标题
氯化镁胁迫对 psi- 酵母细胞中 Sup35p 聚集的定量影响。
DOI：
10.2174/0929866511009011489
复制
发表时间：
2010-11
影响因子：
1.6
通讯作者：
Ben S.
中科院分区：
文献类型：
--
作者：
宋有涛;Song Y.;Lan W.;Wu X.;He J.;Li H.;Ben S.
[PSI(在SUP 35重复扩增突变株[psi（-）]中，以MgCl（2）为选择压时，可瞬时诱导[psi（+）]表型。我们进一步研究了在不同MgCl（2）条件下天然Sup 35 p的[PSI（+）]表型变化，并通过光漂白后荧光恢复（FRAP）和半变性洗涤剂琼脂糖凝胶电泳（SDD-AGE）分析定量Sup 35 p状态变化。结果发现，[PSI（+）]表型与MgCl（2）浓度呈剂量依赖关系。此外，Sup 35 p在MgCl（2）处理的细胞中聚集，但不像在[PSI（+）]细胞中那样形成大的聚集体，并且Sup 35 p聚集体的大小显示出与MgCl（2）应用的时间依赖性关系。Sup 35 p的聚集严格取决于我们菌株中MgCl（2）应激的存在。
[PSI(+)] phenotype can be transiently induced when Magnesium chloride (MgCl(2)) was the selective pressure in SUP35 repeat-expansion mutant [psi(-)] yeast strains. We further investigated [PSI(+)] phenotype change under different MgCl(2) conditions with native Sup35p and quantified the Sup35p status changes with fluorescence recovery after photobleaching (FRAP) and semi-denaturing detergent-agarose gel electrophoresis (SDD-AGE) analysis. It was found that the [PSI(+)] phenotype presented a dose-dependent relationship with the concentrations of MgCl(2). Furthermore, Sup35p aggregated in MgCl(2) treated cells but did not form large aggregates as it does in [PSI(+)] cells, and the size of Sup35p aggregates showed a time-dependent relationship with the MgCl(2) application. The aggregation of Sup35p strictly depended on the presence of MgCl(2) stress in our strains.