Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon:: Seasonal expression profile in pituitary and ovary of maturing female broodstock
Cloning of somatolactin alpha, beta forms and the somatolactin receptor in Atlantic salmon:: Seasonal expression profile in pituitary and ovary of maturing female broodstock
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DOI：
10.1186/1477-7827-6-42
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发表时间：
2008-09-15
影响因子：
4.4
通讯作者：
Andersson, Eva
中科院分区：
文献类型：
--
作者：
Benedet, Susana;Bjornsson, Bjorn Thrandur;Andersson, Eva
背景：生长激素（Somatolactin，Sl）是一种与生长激素（growth hormone，Gh）相关的鱼类特异性腺垂体肽类激素。一些物种，包括鲑鱼，具有两种形式：Sl α和Sl β。生长激素受体与生长激素受体密切相关。SL具有许多生理功能，包括在性成熟中的作用。为了阐明Sl在雌性大西洋鲑（萨尔莫salar）性成熟中的作用，本研究克隆了slr、Sl α和Sl β的全长cDNA，并利用实时荧光定量PCR（RTqPCR）技术研究了它们在大西洋鲑生殖周期中的表达。使用RTqPCR在17个月的时间内研究了Sl α、SL β和slr的基因表达，所述时间包括鲑鱼雌性的卵黄发生前、卵黄发生、排卵和排卵后。卵巢样本的组织学检查允许的分类，根据卵泡成熟成油滴，初级，次级或三级yolk stage.Results的程度：Sl α，Sl β和SLR的成熟肽序列是高度相似的，以前克隆的鲑鱼的形式，并包含典型的基序。大西洋鲑鱼Sl α和Sl β的系统发育分析表明，这些肽分为存在于一些鱼类物种中的两个Sl分支。大西洋鲑鱼slr分组与鲑鱼slr之间所谓的I型生长激素受体。垂体Sl α和Sl β转录物在产卵前和产卵期间增加，排卵后减少，卵巢SLR的表达水平恒定。5月份，在从海水转移到淡水并随后禁食之前，Sl α和Sl β也出现短暂增加。结论：Sl α和Sl β在卵黄发生和产卵期间上调，随后在排卵后下降，支持Sl在性腺生长和产卵期间的作用。S1也可能参与与卵黄发生相关的钙/磷酸盐动员，或在禁食期间与脂解相关的能量稳态中发挥作用。在禁食和淡水转移之前Sl α和Sl β的上调表明Sl与生殖相关的作用可能独立于成熟诱导的禁食。
Background: Somatolactin (Sl) is a fish specific adenohypophyseal peptide hormone related to growth hormone (Gh). Some species, including salmonids, possess two forms: Sl alpha and Sl beta. The somatolactin receptor (slr) is closely related to the growth hormone receptor (ghr). Sl has been ascribed many physiological functions, including a role in sexual maturation. In order to clarify the role of Sl in the sexual maturation of female Atlantic salmon (Salmo salar), the full length cDNAs of slr, Sl alpha and Sl beta were cloned and their expression was studied throughout a seasonal reproductive cycle using real-time quantitative PCR (RTqPCR).Methods: Atlantic salmon Sl alpha, Sl beta and slr cDNAs were cloned using a PCR approach. Gene expression of Sl alpha, SL beta and slr was studied using RTqPCR over a 17 month period encompassing pre-vitellogenesis, vitellogenesis, ovulation and post ovulation in salmon females. Histological examination of ovarian samples allowed for the classification according to the degree of follicle maturation into oil drop, primary, secondary or tertiary yolk stage.Results: The mature peptide sequences of Sl alpha, Sl beta and slr are highly similar to previously cloned salmonid forms and contained the typical motifs. Phylogenetic analysis of Atlantic salmon Sl alpha and Sl beta shows that these peptides group into the two Sl clades present in some fish species. The Atlantic salmon slr grouped with salmonid slr amongst so-called type I ghr. An increase in pituitary Sl alpha and Sl beta transcripts before and during spawning, with a decrease post-ovulation, and a constant expression level of ovarian slr were observed. There was also a transient increase in Sl alpha and Sl beta in May prior to transfer from seawater to fresh water and ensuing fasting.Conclusion: The up-regulation of Sl alpha and Sl beta during vitellogenesis and spawning, with a subsequent decrease post-ovulation, supports a role for Sl during gonadal growth and spawning. Sl could also be involved in calcium/phosphate mobilization associated with vitellogenesis or have a role in energy homeostasis associated with lipolysis during fasting. The up-regulation of both Sl alpha and Sl beta prior to fasting and freshwater transfer, suggests a role for Sl linked to reproduction that may be independent of the maturation induced fasting.